The Library
Mitogenic factor (MF) is the major DNase of serotype M89 Streptococcus pyogenes.
Tools
Sriskandan, Shiranee, Unnikrishnan, Meera, Krausz, Thomas and Cohen, Jonathan (2000) Mitogenic factor (MF) is the major DNase of serotype M89 Streptococcus pyogenes. Microbiology, Volume 146 (Number 11). pp. 2785-2792. ISSN 1350-0872.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://mic.sgmjournals.org/content/146/11/2785.lon...
Abstract
To investigate the role of mitogenic factor (MF) in streptococcal pathogenesis, the structural gene (mf) encoding this protein was disrupted in a clinical isolate of Streptococcus pyogenes H293, to yield the isogenic mutant H363. Growth in enriched broth and on blood agar was unaffected by disruption of mf. Cell-free broth supernatants from H293 and H363 demonstrated identical promitogenic activities when co-incubated with human peripheral blood mononuclear cells, even when diluted 100000-fold, showing that MF is not a major streptococcal mitogen compared with other secreted superantigens. Disruption of mf resulted in complete loss of DNase B production and detectable DNase activity in H363 compared with the parent strain, confirming that the single gene mf, which is present in all group A streptococcal M serotypes studied, encodes DNase B. Despite loss of DNase activity, the virulence of S. pyogenes in a mouse model of necrotizing fasciitis and myositis was unaffected.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Subjects: | Q Science > QP Physiology Q Science > QR Microbiology R Medicine > RC Internal medicine |
||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
||||
Library of Congress Subject Headings (LCSH): | Streptococcus pyogenes, Mitogens, Deoxyribonucleases, Necrotizing fasciitis -- Animal models | ||||
Journal or Publication Title: | Microbiology | ||||
Publisher: | Society for General Microbiology | ||||
ISSN: | 1350-0872 | ||||
Official Date: | November 2000 | ||||
Dates: |
|
||||
Volume: | Volume 146 | ||||
Number: | Number 11 | ||||
Page Range: | pp. 2785-2792 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Funder: | Medical Research Council (Great Britain) (MRC) | ||||
Grant number: | G108/245 (MRC) |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |