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The mouse tumor cell lines EL4 and RMA display mosaic expression of NK-related and certain other surface molecules and appear to have a common origin
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Gays, Frances, Unnikrishnan, Meera, Shrestha, Sunil, Fraser, Karen P., Brown, Adam R., Tristram, Colin M. G., Chrzanowska-Lightowlers, Z. M. and Brooks, Colin G. (2000) The mouse tumor cell lines EL4 and RMA display mosaic expression of NK-related and certain other surface molecules and appear to have a common origin. Journal of Immunology, Volume 164 (Number 10). pp. 5094-5102. ISSN 0022-1767.
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Official URL: http://www.jimmunol.org/content/164/10/5094.long
Abstract
As a potential means for facilitating studies of NK cell-related molecules, we examined the expression of these molecules on a range of mouse tumor cell lines. Of the lines we initially examined, only EL4 and RMA expressed such molecules, both lines expressing several members of the Ly49 and NKRP1 families. Unexpectedly, several of the NK-related molecules, together with certain other molecules including CD2, CD3, CD4, CD32, and CD44, were often expressed in a mosaic manner, even on freshly derived clones, indicating frequent switching in expression. In each case examined, switching was controlled at the mRNA level, with expression of CD3zeta determining expression of the entire CD3-TCR complex. Each of the variable molecules was expressed independently, with the exception that CD3 was restricted to cells that also expressed CD2. Treatment with drugs that affect DNA methylation and histone acetylation could augment the expression of at least some of the variable molecules. The striking phenotypic similarity between EL4 and RMA led us to examine the state of their TCRbeta genes. Both lines had identical rearrangements on both chromosomes, indicating that RMA is in fact a subline of EL4. Overall, these findings suggest that EL4 is an NK-T cell tumor that may have retained a genetic mechanism that permits the variable expression of a restricted group of molecules involved in recognition and signaling.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QH Natural history > QH426 Genetics Q Science > QR Microbiology > QR180 Immunology |
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Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Library of Congress Subject Headings (LCSH): | Killer cells, Mosaic viruses, Gene expression, Cancer cells, Mice -- Diseases -- Research | ||||
Journal or Publication Title: | Journal of Immunology | ||||
Publisher: | American Association of Immunologists | ||||
ISSN: | 0022-1767 | ||||
Official Date: | 15 May 2000 | ||||
Dates: |
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Volume: | Volume 164 | ||||
Number: | Number 10 | ||||
Page Range: | pp. 5094-5102 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Funder: | University of Newcastle, Medical Research Council (Great Britain) (MRC), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Cancer Research UK (CRUK) |
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