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Monopolin subunit Csm1 associates with MIND complex to establish monopolar attachment of sister kinetochores at meiosis I
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Sarkar, Sourav, Shenoy, Rajesh T., Dalgaard, Jacob Z., Newnham, Louise, Hoffmann, Eva, Millar, Jonathan B. A. and Arumugam, Prakash (2013) Monopolin subunit Csm1 associates with MIND complex to establish monopolar attachment of sister kinetochores at meiosis I. PLoS Genetics, Volume 9 (Number 7). Article number e1003610. doi:10.1371/journal.pgen.1003610 ISSN 1553-7390.
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WRAP_Sarkar_journal.pgen.1003610.pdf - Published Version Available under License Creative Commons Attribution. Download (2215Kb) | Preview |
Official URL: http://dx.doi.org/10.1371/journal.pgen.1003610
Abstract
Sexually reproducing organisms halve their cellular ploidy during gametogenesis by undergoing a specialized form of cell division known as meiosis. During meiosis, a single round of DNA replication is followed by two rounds of nuclear divisions (referred to as meiosis I and II). While sister kinetochores bind to microtubules emanating from opposite spindle poles during mitosis, they bind to microtubules originating from the same spindle pole during meiosis I. This phenomenon is referred to as mono-orientation and is essential for setting up the reductional mode of chromosome segregation during meiosis I. In budding yeast, mono-orientation depends on a four component protein complex referred to as monopolin which consists of two nucleolar proteins Csm1 and Lrs4, meiosis-specific protein Mam1 of unknown function and casein kinase Hrr25. Monopolin complex binds to kinetochores during meiosis I and prevents bipolar attachments. Although monopolin associates with kinetochores during meiosis I, its binding site(s) on the kinetochore is not known and its mechanism of action has not been established. By carrying out an imaging-based screen we have found that the MIND complex, a component of the central kinetochore, is required for monopolin association with kinetochores during meiosis. Furthermore, we demonstrate that interaction of monopolin subunit Csm1 with the N-terminal domain of MIND complex subunit Dsn1, is essential for both the association of monopolin with kinetochores and for monopolar attachment of sister kinetochores during meiosis I. As such this provides the first functional evidence for a monopolin-binding site at the kinetochore.
| Item Type: | Journal Article | ||||
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| Subjects: | Q Science > QH Natural history > QH301 Biology | ||||
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Meiosis, Nuclear proteins | ||||
| Journal or Publication Title: | PLoS Genetics | ||||
| Publisher: | Public Library of Science | ||||
| ISSN: | 1553-7390 | ||||
| Official Date: | 4 July 2013 | ||||
| Dates: |
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| Volume: | Volume 9 | ||||
| Number: | Number 7 | ||||
| Page Range: | Article number e1003610 | ||||
| DOI: | 10.1371/journal.pgen.1003610 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||
| Date of first compliant deposit: | 26 December 2015 | ||||
| Date of first compliant Open Access: | 26 December 2015 | ||||
| Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Wellcome Trust (London, England) | ||||
| Grant number: | BB/G00353X/1b (BBSRC) |
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