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Retinal proteins as model systems for membrane protein folding
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Tastan, Oznur, Dutta, Arpana, Booth, Paula and Klein-Seetharaman, Judith (2014) Retinal proteins as model systems for membrane protein folding. Biochimica et Biophysica Acta (BBA) - Bioenergetics, Volume 1837 (Number 5). pp. 656-663. doi:10.1016/j.bbabio.2013.11.021 ISSN 0005-2728.
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Official URL: http://dx.doi.org/10.1016/j.bbabio.2013.11.021
Abstract
Experimental folding studies of membrane proteins are more challenging than water-soluble proteins because of the higher hydrophobicity content of membrane embedded sequences and the need to provide a hydrophobic milieu for the transmembrane regions. The first challenge is their denaturation: due to the thermodynamic instability of polar groups in the membrane, secondary structures in membrane proteins are more difficult to disrupt than in soluble proteins. The second challenge is to refold from the denatured states. Successful refolding of membrane proteins has almost always been from very subtly denatured states. Therefore, it can be useful to analyze membrane protein folding using computational methods, and we will provide results obtained with simulated unfolding of membrane protein structures using the Floppy Inclusions and Rigid Substructure Topography (FIRST) method. Computational methods have the advantage that they allow a direct comparison between diverse membrane proteins. We will review here both, experimental and FIRST studies of the retinal binding proteins bacteriorhodopsin and mammalian rhodopsin, and discuss the extension of the findings to deriving hypotheses on the mechanisms of folding of membrane proteins in general. This article is part of a Special Issue entitled: Retinal Proteins—You can teach an old dog new tricks.
Item Type: | Journal Article | ||||||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Metabolic and Vascular Health (- until July 2016) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine |
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Journal or Publication Title: | Biochimica et Biophysica Acta (BBA) - Bioenergetics | ||||||||||
Publisher: | Elsevier Science BV | ||||||||||
ISSN: | 0005-2728 | ||||||||||
Official Date: | May 2014 | ||||||||||
Dates: |
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Volume: | Volume 1837 | ||||||||||
Number: | Number 5 | ||||||||||
Page Range: | pp. 656-663 | ||||||||||
DOI: | 10.1016/j.bbabio.2013.11.021 | ||||||||||
Status: | Peer Reviewed | ||||||||||
Publication Status: | Published | ||||||||||
Access rights to Published version: | Restricted or Subscription Access |
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