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Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats
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Dealtry, Simone, Ding, Guo-Chun, Weichelt, Viola, Dunon, Vincent, Schlüter, Andreas, Martini, María Carla, Papa, María Florencia Del, Lagares, Antonio, Amos, Gregory C. A., Wellington, E. M. H., Gaze, William H., Sipkema, Detmer, Sjöling, Sara, Springael, Dirk, Heuer, Holger, Elsas, J. D. van, Thomas, Christopher M. and Smalla, Kornelia (2014) Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats. PLoS One, Volume 9 (Number 2). Article number e89922. doi:10.1371/journal.pone.0089922 ISSN 1932-6203.
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Official URL: http://dx.doi.org/10.1371/journal.pone.0089922
Abstract
IncP-1, IncP-7 and IncP-9 plasmids often carry genes encoding enzymes involved in the degradation of man-made and natural contaminants, thus contributing to bacterial survival in polluted environments. However, the lack of suitable molecular tools often limits the detection of these plasmids in the environment. In this study, PCR followed by Southern blot hybridization detected the presence of plasmid-specific sequences in total community (TC-) DNA or fosmid DNA from samples originating from different environments and geographic regions. A novel primer system targeting IncP-9 plasmids was developed and applied along with established primers for IncP-1 and IncP-7. Screening TC-DNA from biopurification systems (BPS) which are used on farms for the purification of pesticide-contaminated water revealed high abundances of IncP-1 plasmids belonging to different subgroups as well as IncP-7 and IncP-9. The novel IncP-9 primer-system targeting the rep gene of nine IncP-9 subgroups allowed the detection of a high diversity of IncP-9 plasmid specific sequences in environments with different sources of pollution. Thus polluted sites are "hot spots" of plasmids potentially carrying catabolic genes.
Item Type: | Journal Article | ||||||
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Subjects: | Q Science > QH Natural history > QH426 Genetics | ||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
Library of Congress Subject Headings (LCSH): | Microbial genetics | ||||||
Journal or Publication Title: | PLoS One | ||||||
Publisher: | Public Library of Science | ||||||
ISSN: | 1932-6203 | ||||||
Official Date: | 24 February 2014 | ||||||
Dates: |
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Volume: | Volume 9 | ||||||
Number: | Number 2 | ||||||
Article Number: | Article number e89922 | ||||||
DOI: | 10.1371/journal.pone.0089922 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||
Date of first compliant deposit: | 26 December 2015 | ||||||
Date of first compliant Open Access: | 26 December 2015 | ||||||
Funder: | Seventh Framework Programme (European Commission) (FP7), Belgium. Politique scientifique fédérale [Belgian State Science Policy Office] (BELSPO) | ||||||
Grant number: | MetaExplore 222625 (FP7), P7/25 (BELSPO) |
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