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Quantitative investigation of calcimimetic R568 on beta cell adhesion and mechanics using AFM single-cell force spectroscopy

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Siamantouras, Eleftherios, Hills, Claire E., Younis, Mustafa Y. G., Squires, Paul E. and Liu, Kuo-Kang (2014) Quantitative investigation of calcimimetic R568 on beta cell adhesion and mechanics using AFM single-cell force spectroscopy. FEBS Letters, Volume 588 (Number 7). pp. 1173-1178. doi:10.1016/j.febslet.2014.02.058 ISSN 0014-5793.

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Official URL: http://dx.doi.org/10.1016/j.febslet.2014.02.058

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Abstract

In this study we use a novel approach to quantitatively investigate mechanical and interfacial properties of clonal β-cells using AFM-Single Cell Force Spectroscopy (SCFS). MIN6 cells were incubated for 48hrs with 0.5mM Ca2+ ± the calcimimetic R568 (1μM). AFM-SCFS adhesion and indentation experiments were performed by using modified tipless cantilevers. Hertz contact model was applied to analyse force-displacement (F-d) curves for determining elastic or Young's modulus (E). Our results show CaSR-evoked increases in cell-to-cell adhesion parameters and E modulus of single cells, demonstrating that cytomechanics have profound effects on cell adhesion characterization.

Item Type: Journal Article
Subjects: Q Science > QP Physiology
T Technology > TJ Mechanical engineering and machinery
Divisions: Faculty of Science, Engineering and Medicine > Engineering > Engineering
Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH): Pancreatic beta cells, Cell adhesion, Calcium -- Receptors
Journal or Publication Title: FEBS Letters
Publisher: Elsevier BV
ISSN: 0014-5793
Official Date: 2 April 2014
Dates:
DateEvent
2 April 2014Published
Volume: Volume 588
Number: Number 7
Number of Pages: 6
Page Range: pp. 1173-1178
DOI: 10.1016/j.febslet.2014.02.058
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
Date of first compliant deposit: 26 December 2015
Date of first compliant Open Access: 26 December 2015
Funder: Diabetes UK, Leverhulme Trust (LT)
Grant number: BDA:09/0003913 (DUK), BDA: 12/0004546 (DUK), PRG-2012-738 (LT)
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