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Quantitative investigation of calcimimetic R568 on beta cell adhesion and mechanics using AFM single-cell force spectroscopy
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Siamantouras, Eleftherios, Hills, Claire E., Younis, Mustafa Y. G., Squires, Paul E. and Liu, Kuo-Kang (2014) Quantitative investigation of calcimimetic R568 on beta cell adhesion and mechanics using AFM single-cell force spectroscopy. FEBS Letters, Volume 588 (Number 7). pp. 1173-1178. doi:10.1016/j.febslet.2014.02.058 ISSN 0014-5793.
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WRAP_Squires_Febs Lett-2014-CaR %28v9%29.pdf - Accepted Version - Requires a PDF viewer. Download (1092Kb) | Preview |
Official URL: http://dx.doi.org/10.1016/j.febslet.2014.02.058
Abstract
In this study we use a novel approach to quantitatively investigate mechanical and interfacial properties of clonal β-cells using AFM-Single Cell Force Spectroscopy (SCFS). MIN6 cells were incubated for 48hrs with 0.5mM Ca2+ ± the calcimimetic R568 (1μM). AFM-SCFS adhesion and indentation experiments were performed by using modified tipless cantilevers. Hertz contact model was applied to analyse force-displacement (F-d) curves for determining elastic or Young's modulus (E). Our results show CaSR-evoked increases in cell-to-cell adhesion parameters and E modulus of single cells, demonstrating that cytomechanics have profound effects on cell adhesion characterization.
| Item Type: | Journal Article | ||||
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| Subjects: | Q Science > QP Physiology T Technology > TJ Mechanical engineering and machinery |
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| Divisions: | Faculty of Science, Engineering and Medicine > Engineering > Engineering Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) |
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| Library of Congress Subject Headings (LCSH): | Pancreatic beta cells, Cell adhesion, Calcium -- Receptors | ||||
| Journal or Publication Title: | FEBS Letters | ||||
| Publisher: | Elsevier BV | ||||
| ISSN: | 0014-5793 | ||||
| Official Date: | 2 April 2014 | ||||
| Dates: |
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| Volume: | Volume 588 | ||||
| Number: | Number 7 | ||||
| Number of Pages: | 6 | ||||
| Page Range: | pp. 1173-1178 | ||||
| DOI: | 10.1016/j.febslet.2014.02.058 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Date of first compliant deposit: | 26 December 2015 | ||||
| Date of first compliant Open Access: | 26 December 2015 | ||||
| Funder: | Diabetes UK, Leverhulme Trust (LT) | ||||
| Grant number: | BDA:09/0003913 (DUK), BDA: 12/0004546 (DUK), PRG-2012-738 (LT) | ||||
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