The Library
Estimation of antibody binding affinities with surface plasmon resonance experiments in incompatible blood type renal transplants
Tools
Tools
Tools
Moyse, Harry, Lowe, David Philip, Briggs, D., Higgins, Rob, Bentall, A., Ball, S., Mitchell, Daniel A., Chappell, M. J. (Michael J.), Zehnder, Daniel and Evans, Neil D. (2012) Estimation of antibody binding affinities with surface plasmon resonance experiments in incompatible blood type renal transplants. In: 8th IFAC Symposium on Biological and Medical Systems, Budapest, Hungary, 29-31 Aug 2012. Published in: Biological and Medical Systems, Volume 8 (Number 1). pp. 97-102. ISBN 9783902823106. doi:10.3182/20120829-3-HU-2029.00088 ISSN 1474-6670.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.3182/20120829-3-HU-2029.00088
Abstract
Blood group (ABO) incompatible transplants carry an increased risk of rejection. This risk could be dramatically reduced by the removal and suppression of the antibody types that would attack the donor organ. A prerequisite for this removal is an experimental procedure that can estimate the binding affinities of multiple antibodies from patient blood samples. This paper presents the usage of surface plasmon resonance (SPR) experiments with a pre existing mathematical model and a recently created expanded version of it that can estimate multiple antibody binding affinities from parallel experiments. SPR experiments were conducted on purified patient antibody samples of the IgG and IgA isotype, as well as mixed antibody from the other isotypes. The result of these experiments was analyzed with both mathematical models, and the expanded model was demonstrated to give a vastly improved fit. Estimates of antibody binding affinity were compared between samples and the non IgG/IgA protein sample was seen to have the highest binding affinity.
| Item Type: | Conference Item (Paper) | ||||
|---|---|---|---|---|---|
| Divisions: | Faculty of Science, Engineering and Medicine > Engineering > Engineering Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Metabolic and Vascular Health (- until July 2016) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
||||
| Journal or Publication Title: | Biological and Medical Systems | ||||
| Publisher: | IFAC Secretariat | ||||
| ISBN: | 9783902823106 | ||||
| ISSN: | 1474-6670 | ||||
| Book Title: | 8th IFAC Symposium on Biological and Medical Systems (2012) | ||||
| Official Date: | 2012 | ||||
| Dates: |
|
||||
| Volume: | Volume 8 | ||||
| Number: | Number 1 | ||||
| Page Range: | pp. 97-102 | ||||
| DOI: | 10.3182/20120829-3-HU-2029.00088 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Conference Paper Type: | Paper | ||||
| Title of Event: | 8th IFAC Symposium on Biological and Medical Systems | ||||
| Type of Event: | Other | ||||
| Location of Event: | Budapest, Hungary | ||||
| Date(s) of Event: | 29-31 Aug 2012 | ||||
| Related URLs: |
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
