The Library
Synthetic polymers enable non-vitreous cellular cryopreservation by reducing ice crystal growth during thawing
Tools
Deller, Robert C., Vatish, Manu, Mitchell, Daniel A. and Gibson, Matthew I. (2014) Synthetic polymers enable non-vitreous cellular cryopreservation by reducing ice crystal growth during thawing. Nature Communications, Volume 5 . Article number 3244. doi:10.1038/ncomms4244 ISSN 2041-1723.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1038/ncomms4244
Abstract
The cryopreservation of cells, tissue and organs is fundamental to modern biotechnology, transplantation medicine and chemical biology. The current state-of-the-art method of cryopreservation is the addition of large amounts of organic solvents such as glycerol or dimethyl sulfoxide, to promote vitrification and prevent ice formation. Here we employ a synthetic, biomimetic, polymer, which is capable of slowing the growth of ice crystals in a manner similar to antifreeze (glyco)proteins to enhance the cryopreservation of sheep and human red blood cells. We find that only 0.1 wt% of the polymer is required to attain significant cell recovery post freezing, compared with over 20 wt% required for solvent-based strategies. These results demonstrate that synthetic antifreeze (glyco)protein mimics could have a crucial role in modern regenerative medicine to improve the storage and distribution of biological material for transplantation.
Item Type: | Journal Article | ||||||||
---|---|---|---|---|---|---|---|---|---|
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Research Centres > Molecular Organisation and Assembly in Cells (MOAC) |
||||||||
Journal or Publication Title: | Nature Communications | ||||||||
Publisher: | Nature Publishing Group | ||||||||
ISSN: | 2041-1723 | ||||||||
Official Date: | 3 February 2014 | ||||||||
Dates: |
|
||||||||
Volume: | Volume 5 | ||||||||
Article Number: | Article number 3244 | ||||||||
DOI: | 10.1038/ncomms4244 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Restricted or Subscription Access |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |