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Methods, challenges and potentials of single cell RNA-seq
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Hebenstreit, Daniel (2012) Methods, challenges and potentials of single cell RNA-seq. Biology, Volume 1 (Number 3). pp. 658-667. doi:10.3390/biology1030658 ISSN 2079-7737.
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Official URL: http://dx.doi.org/10.3390/biology1030658
Abstract
RNA-sequencing (RNA-seq) has become the tool of choice for transcriptomics. Several recent studies demonstrate its successful adaption to single cell analysis. This allows new biological insights into cell differentiation, cell-to-cell variation and gene regulation, and how these aspects depend on each other. Here, I review the current single cell RNA-seq (scRNA-seq) efforts and discuss experimental protocols, challenges and potentials.
Item Type: | Journal Article | ||||||||
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Subjects: | Q Science > QH Natural history > QH426 Genetics | ||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
Library of Congress Subject Headings (LCSH): | Nucleotide sequence, Genetic transcription | ||||||||
Journal or Publication Title: | Biology | ||||||||
Publisher: | M D P I AG | ||||||||
ISSN: | 2079-7737 | ||||||||
Official Date: | 16 November 2012 | ||||||||
Dates: |
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Volume: | Volume 1 | ||||||||
Number: | Number 3 | ||||||||
Page Range: | pp. 658-667 | ||||||||
DOI: | 10.3390/biology1030658 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||
Date of first compliant deposit: | 27 December 2015 | ||||||||
Date of first compliant Open Access: | 27 December 2015 |
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