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Absorption-mode Fourier transform mass spectrometry : the effects of apodization and phasing on modified protein spectra
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Qi, Yulin, Li, Huilin, Wills, Rebecca H., Perez-Hurtado, Pilar, Yu, Xiang, Kilgour, David P. A., Barrow, Mark P., Lin, Cheng and O’Connor, Peter B. (2013) Absorption-mode Fourier transform mass spectrometry : the effects of apodization and phasing on modified protein spectra. Journal of The American Society for Mass Spectrometry, Volume 24 (Number 6). pp. 828-834. doi:10.1007/s13361-013-0600-6 ISSN 1044-0305.
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Official URL: http://dx.doi.org/10.1007/s13361-013-0600-6
Abstract
The method of phasing broadband Fourier transform ion cyclotron resonance (FT-ICR) spectra allows plotting the spectra in the absorption-mode; this new approach significantly improves the quality of the data at no extra cost. Herein, an internal calibration method for calculating the phase function has been developed and successfully applied to the top-down spectra of modified proteins, where the peak intensities vary by 100×. The result shows that the use of absorption-mode spectra allows more peaks to be discerned within the recorded data, and this can reveal much greater information about the protein and modifications under investigation. In addition, noise and harmonic peaks can be assigned immediately in the absorption-mode.
Item Type: | Journal Article | ||||||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||||||||
Journal or Publication Title: | Journal of The American Society for Mass Spectrometry | ||||||||||
Publisher: | Springer New York LLC | ||||||||||
ISSN: | 1044-0305 | ||||||||||
Official Date: | June 2013 | ||||||||||
Dates: |
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Volume: | Volume 24 | ||||||||||
Number: | Number 6 | ||||||||||
Page Range: | pp. 828-834 | ||||||||||
DOI: | 10.1007/s13361-013-0600-6 | ||||||||||
Status: | Peer Reviewed | ||||||||||
Publication Status: | Published | ||||||||||
Access rights to Published version: | Restricted or Subscription Access |
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