Carter, Anthony D., Bonyadi, Roxanna and Gifford, Miriam L. (2013) The use of fluorescence-activated cell sorting in studying plant development and environmental responses. The International Journal of Developmental Biology, Volume 57 . pp. 545-552. doi:10.1387/ijdb.130195mg ISSN 0214-6282.

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Abstract

Fluorescence-Activated Cell Sorting (FACS) is a powerful tool that enables plant growth and development to be studied at the cellular level. Flow cytometry is used to isolate subpopulations of cells, such as those of specific cell types, or cells at particular developmental stages that have been marked with fluorescent proteins. Transgenic technology has given us the ability to generate plants that express fluorescent proteins, not just constitutively in particular cell types, but also dynamically in response to endogenous or external factors. By processing such transgenic lines with FACS, it is possible to isolate distinct populations of cells in a wide range of likely response states for further analysis. This is particularly useful for investigating biological mechanisms in plants because the control of growth and development is manifest at the cell type level. Furthermore, the specificity of the resulting data enables fine modelling of the transcriptional networks that exert systems-level control of the transcriptome; hence key regulators of responses and processes in the plant can be identified. In this review, the current state of the art for FACS methods in plants is explored by means of case studies of research in which cell sorting allowed us to make significant new discoveries.

Item Type:	Journal Article
Subjects:	Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics Q Science > QK Botany S Agriculture > SB Plant culture
Divisions:	Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH):	Roots (Botany), Arabidopsis, Cell separation, Flow cytometry, Plant genetic engineering
Journal or Publication Title:	The International Journal of Developmental Biology
Publisher:	Universidad del Pais Vasco * Servicio Editorial
ISSN:	0214-6282
Official Date:	6 September 2013
Dates:	Date Event 6 September 2013 Published
Volume:	Volume 57
Page Range:	pp. 545-552
DOI:	10.1387/ijdb.130195mg
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Open Access (Creative Commons)
Date of first compliant deposit:	27 December 2015
Date of first compliant Open Access:	27 December 2015
Funder:	Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Engineering and Physical Sciences Research Council (EPSRC), University of Warwick Postgraduate Research Scholarship, Stapley Educational Trust grant
Grant number:	BB/H109502/1 (BBSRC)

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