Davenport, Rebecca, Heawood, Christopher, Sessford, Kate, Baker, Melissa, Baiker, Kerstin, Blacklaws, Barbara, Kaler, Jasmeet, Green, Laura E. and Tötemeyer, Sabine (2014) Differential expression of Toll-like receptors and inflammatory cytokines in ovine interdigital dermatitis and footrot. Veterinary Immunology and Immunopathology, Volume 161 (Number 1–2). pp. 90-98. doi:10.1016/j.vetimm.2014.07.007 ISSN 0165-2427.

Preview

PDF WRAP_Green_1-s2.0-S0165242714001706-main.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution. Download (2527Kb) | Preview

Request Changes to record.

Abstract

Footrot is a common inflammatory bacterial disease affecting the health and welfare of sheep worldwide. The pathogenesis of footrot is complex and multifactorial. The primary causal pathogen is the anaerobic bacterium Dichelobacter nodosus, with Fusobacterium necrophorum also shown to play a key role in disease. Since immune-mediated pathology is implicated, the aim of this research was to investigate the role of the host response in interdigital dermatitis (ID) and footrot. We compared the expression of Toll-like receptors (TLR) and pro-inflammatory cytokines and the histological appearance of clinically normal in comparison to ID and footrot affected tissues. Severe ID and footrot were characterized by significantly increased transcript levels of pro-inflammatory cytokines TNFα and IL1β and the pattern recognition receptors TLR2 and TLR4 in the interdigital skin. This was reflected in the histopathological appearance, with ID and footrot presenting progressive chronic-active pododermatitis with a mixed lymphocytic and neutrophilic infiltration, gradually increasing from a mild form in clinically normal feet, to moderate in ID and to a focally severe form with frequent areas of purulence in footrot. Stimulation with F. necrophorum and/or D. nodosus extracts demonstrated that dermal fibroblasts, the resident cell type of the dermis, also contribute to the inflammatory response to footrot bacteria by increased expression of TNFα, IL1β and TLR2. Overall, ID and footrot lead to a local inflammatory response given that expression levels of TLRs and IL1β were dependent on the disease state of the foot not the animal.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology > QR180 Immunology S Agriculture > SF Animal culture
Divisions:	Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH):	Veterinary Medicine, Footrot in sheep
Journal or Publication Title:	Veterinary Immunology and Immunopathology
Publisher:	Elsevier Science BV
ISSN:	0165-2427
Official Date:	15 September 2014
Dates:	Date Event 15 September 2014 Published 24 July 2014 Available 17 July 2014 Accepted 28 March 2014 Submitted
Volume:	Volume 161
Number:	Number 1–2
Page Range:	pp. 90-98
DOI:	10.1016/j.vetimm.2014.07.007
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Open Access (Creative Commons)
Date of first compliant deposit:	27 December 2015
Date of first compliant Open Access:	27 December 2015
Funder:	University of Nottingham, Wellcome Trust (London, England), MSD Animal Health

Request changes or add full text files to a record

Repository staff actions (login required)

View Item

Downloads