The Library
Conjugative interaction induces transposition of ISPst9 in Pseudomonas stutzeri AN10
Tools
Christie-Oleza, Joseph Alexander, Lanfranconi, M. P., Nogales, B., Lalucat, J. and Bosch, R. (2009) Conjugative interaction induces transposition of ISPst9 in Pseudomonas stutzeri AN10. Journal of Bacteriology, Volume 191 (Number 4). pp. 1239-1247. doi:10.1128/JB.01071-08 ISSN 0021-9193.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1128/JB.01071-08
Abstract
ISPst9 is an ISL3-like insertion sequence (IS) that was recently described in the naphthalene-degrading organism Pseudomonas stutzeri strain AN10. In this paper we describe a novel strong IS regulation stimulus; transposition of ISPst9 is induced in all P. stutzeri AN10 cells after conjugative interaction with Escherichia coli. Thus, we observed that in all P. stutzeri AN10 cells that received genetic material by conjugation the ISPst9 genomic dose and/or distribution was changed. Furthermore, ISPst9 transposition was also observed when P. stutzeri AN10 cells were put in contact with the plasmidless conjugative strain E. coli S17-1λpir, but not when they were put in contact with E. coli DH5α (a nonconjugative strain). The mechanism of ISPst9 transposition was analyzed, and transposition was shown to proceed by excision from the donor DNA using a conservative mechanism, which generated 3- to 10-bp deletions of the flanking DNA. Our results indicate that ISPst9 transposes, forming double-stranded DNA circular intermediates consisting of the IS and a 5-bp intervening DNA sequence probably derived from the ISPst9 flanking regions. The kinetics of IS circle formation are also described.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Journal or Publication Title: | Journal of Bacteriology | ||||
Publisher: | American Society for Microbiology | ||||
ISSN: | 0021-9193 | ||||
Official Date: | February 2009 | ||||
Dates: |
|
||||
Volume: | Volume 191 | ||||
Number: | Number 4 | ||||
Page Range: | pp. 1239-1247 | ||||
DOI: | 10.1128/JB.01071-08 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |