The Library
Efficient gene targeting and foreign DNA removal by homologous recombination in the picoeukaryote Ostreococcus
Tools
Tools
Tools
Lozano, Jean-Claude, Schatt, Philippe, Botebol, Hugo, Verge, Valerie, Lesuisse, Emmanuel, Blain, Stephane, Carré, Isabelle A. and Bouget, Francois-Yves (2014) Efficient gene targeting and foreign DNA removal by homologous recombination in the picoeukaryote Ostreococcus. Plant Journal, Volume 78 (Number 6). pp. 1073-1083.
|
PDF
WRAP_Lozano et al.pdf - Accepted Version - Requires a PDF viewer. Download (1388Kb) | Preview |
Official URL: http://dx.doi.org/10.1111/tpj.12530
Abstract
With fewer than 8000 genes and a minimalist cellular organization, the green picoalga Ostreococcus tauri is one of the simplest photosynthetic eukaryotes. Ostreococcus tauri contains many plant-specific genes but exhibits a very low gene redundancy. The haploid genome is extremely dense with few repeated sequences and rare transposons. Thanks to the implementation of genetic transformation and vectors for inducible overexpression/knockdown this picoeukaryotic alga has emerged in recent years as a model organism for functional genomics analyses and systems biology. Here we report the development of an efficient gene targeting technique which we use to knock out the nitrate reductase and ferritin genes and to knock in a luciferase reporter in frame to the ferritin native protein. Furthermore, we show that the frequency of insertion by homologous recombination is greatly enhanced when the transgene is designed to replace an existing genomic insertion. We propose that a natural mechanism based on homologous recombination may operate to remove inserted DNA sequences from the genome.
| Item Type: | Journal Article | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Subjects: | Q Science > Q Science (General) Q Science > QK Botany Q Science > QR Microbiology > QR355 Virology |
||||||||||
| Divisions: | Faculty of Science > Life Sciences (2010- ) | ||||||||||
| Library of Congress Subject Headings (LCSH): | Green algae, Plant cytogenetics, Genetic recombination, Gene targeting, Microalgae, Genetic transformation | ||||||||||
| Journal or Publication Title: | Plant Journal | ||||||||||
| Publisher: | Wiley-Blackwell Publishing Ltd. | ||||||||||
| ISSN: | 0960-7412 | ||||||||||
| Official Date: | June 2014 | ||||||||||
| Dates: |
|
||||||||||
| Date of first compliant deposit: | 28 July 2016 | ||||||||||
| Volume: | Volume 78 | ||||||||||
| Number: | Number 6 | ||||||||||
| Page Range: | pp. 1073-1083 | ||||||||||
| Status: | Peer Reviewed | ||||||||||
| Publication Status: | Published | ||||||||||
| Access rights to Published version: | Restricted or Subscription Access |
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
Downloads
Downloads per month over past year
