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Assessing the exoproteome of marine bacteria, lesson from a RTX-Toxin abundantly secreted by Phaeobacter strain DSM 17395
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Durighello, Emie, Christie-Oleza, Joseph Alexander and Armengaud, Jean (2014) Assessing the exoproteome of marine bacteria, lesson from a RTX-Toxin abundantly secreted by Phaeobacter strain DSM 17395. PLoS One, Volume 9 (Number 2). Article number e89691. doi:10.1371/journal.pone.0089691 ISSN 1932-6203.
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Official URL: http://dx.doi.org/10.1371/journal.pone.0089691
Abstract
Bacteria from the Roseobacter clade are abundant in surface marine ecosystems as over 10% of bacterial cells in the open ocean and 20% in coastal waters belong to this group. In order to document how these marine bacteria interact with their environment, we analyzed the exoproteome of Phaeobacter strain DSM 17395. We grew the strain in marine medium, collected the exoproteome and catalogued its content with high-throughput nanoLC-MS/MS shotgun proteomics. The major component represented 60% of the total protein content but was refractory to either classical proteomic identification or proteogenomics. We de novo sequenced this abundant protein with high-resolution tandem mass spectra which turned out being the 53 kDa RTX-toxin ZP_02147451. It comprised a peptidase M10 serralysin domain. We explained its recalcitrance to trypsin proteolysis and proteomic identification by its unusual low number of basic residues. We found this is a conserved trait in RTX-toxins from Roseobacter strains which probably explains their persistence in the harsh conditions around bacteria. Comprehensive analysis of exoproteomes from environmental bacteria should take into account this proteolytic recalcitrance.
Item Type: | Journal Article | ||||||||
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Subjects: | Q Science > QR Microbiology | ||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
Library of Congress Subject Headings (LCSH): | Marine microbiology, Molecular chaperones , Marine bacteria, Membrane proteins, Serine proteinases, Peptides, Proteomics | ||||||||
Journal or Publication Title: | PLoS One | ||||||||
Publisher: | Public Library of Science | ||||||||
ISSN: | 1932-6203 | ||||||||
Official Date: | 24 February 2014 | ||||||||
Dates: |
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Volume: | Volume 9 | ||||||||
Number: | Number 2 | ||||||||
Article Number: | Article number e89691 | ||||||||
DOI: | 10.1371/journal.pone.0089691 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||
Date of first compliant deposit: | 27 December 2015 | ||||||||
Date of first compliant Open Access: | 27 December 2015 | ||||||||
Funder: | Fundación Ramón Areces, France. Commissariat à l'énergie atomique |
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