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Conformational dynamics of a seven transmembrane helical protein anabaena sensory rhodopsin probed by solid-state NMR
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Good, Daryl B., Wang, Shenlin, Ward, Meaghan E., Struppe, Jochem, Brown, Leonid S., Lewandowski, Józef R. and Ladizhansky, Vladimir (2014) Conformational dynamics of a seven transmembrane helical protein anabaena sensory rhodopsin probed by solid-state NMR. Journal of the American Chemical Society, Volume 136 (Number 7). pp. 2833-2842. doi:10.1021/ja411633w ISSN 0002-7863.
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Official URL: http://dx.doi.org/10.1021/ja411633w
Abstract
The ability to detect and characterize molecular motions represents one of the unique strengths of nuclear magnetic resonance (NMR) spectroscopy. In this study, we report solid-state NMR site-specific measurements of the dipolar order parameters and 15N rotating frame spin–lattice (R1ρ) relaxation rates in a seven transmembrane helical protein Anabaena Sensory Rhodopsin reconstituted in lipids. The magnitudes of the observed order parameters indicate that both the well-defined transmembrane regions and the less structured intramembrane loops undergo restricted submicrosecond time scale motions. In contrast, the R1ρ rates, which were measured under fast magic angle spinning conditions, vary by an order of magnitude between the TM and exposed regions and suggest the presence of intermediate time scale motions. Using a simple model, which assumes a single exponential autocorrelation function, we estimated the time scales of dominant stochastic motions to be on the order of low tens of nanoseconds for most residues within the TM helices and tens to hundreds of nanoseconds for the extracellular B–C and F–G loops. These relatively slow time scales could be attributed to collective anisotropic motions. We used the 3D Gaussian axial fluctuations model to estimate amplitudes, directions, and time scales of overall motions for helices and the extracellular B–C and F–G loops. Within this model, the TM helices A,B,C,D,E,F undergo rigid body motions on a time scale of tens of nanoseconds, while the time scale for the seventh helix G approaches 100 ns. Similar time scales of roughly 100–200 ns are estimated for the B–C and F–G loops.
Item Type: | Journal Article | ||||||||
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Alternative Title: | Conformational dynamics of a seven transmembrane helical protein anabaena sensory rhodopsin probed by solid-state nuclear magnetic resonance | ||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||||||
Journal or Publication Title: | Journal of the American Chemical Society | ||||||||
Publisher: | American Chemical Society | ||||||||
ISSN: | 0002-7863 | ||||||||
Official Date: | 19 February 2014 | ||||||||
Dates: |
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Volume: | Volume 136 | ||||||||
Number: | Number 7 | ||||||||
Page Range: | pp. 2833-2842 | ||||||||
DOI: | 10.1021/ja411633w | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||
Date of first compliant deposit: | 3 February 2017 | ||||||||
Date of first compliant Open Access: | 3 February 2017 |
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