The Library
Purification and characterisation of a non-plant myrosinase from the cabbage aphid Brevicoryne brassicae (L.)
Tools
Jones, Alexandra M., Bridges, M., Bones, A. M., Cole, R. and Rossiter, J. T. (2001) Purification and characterisation of a non-plant myrosinase from the cabbage aphid Brevicoryne brassicae (L.). Insect Biochemistry and Molecular Biology, Volume 31 (Number 1). pp. 1-5. doi:10.1016/S0965-1748(00)00157-0 ISSN 0965-1748.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1016/S0965-1748(00)00157-0
Abstract
Plant myrosinases and glucosinolates constitute a defence system in cruciferous plants towards pests and diseases. We have purified for the first time a non-plant myrosinase from the cabbage aphid Brevicoryne brassicae (L.) to homogeneity. The protein was N-terminally blocked and protease (trypsin and lys c) degradation gave peptides of which five were sequenced. The protein is a dimer with subunits of mass 54 kDa±500 Da. Western blot analysis with an anti-aphid myrosinase antibody showed a strong cross reaction with a protein extract from the Brassica specialist, B. brassicae. The anti-aphid myrosinase antibody does not cross react with plant myrosinase neither does an anti-plant myrosinase antibody cross react with aphid myrosinase.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Journal or Publication Title: | Insect Biochemistry and Molecular Biology | ||||
Publisher: | Pergamon Press | ||||
ISSN: | 0965-1748 | ||||
Official Date: | January 2001 | ||||
Dates: |
|
||||
Volume: | Volume 31 | ||||
Number: | Number 1 | ||||
Page Range: | pp. 1-5 | ||||
DOI: | 10.1016/S0965-1748(00)00157-0 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |