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Exploring the functional soil-microbe interface and exoenzymes through soil metaexoproteomics
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Johnson-Rollings, Ashley S., Wright, H., Masciandaro, Grazia, Macci, Cristina, Doni, Serena, Calvo-Bado, Leo A., Slade, Susan E., Plou, Carlos Vallin and Wellington, E. M. H. (2014) Exploring the functional soil-microbe interface and exoenzymes through soil metaexoproteomics. The ISME Journal, Volume 8 (Number 10). pp. 2148-2150. doi:10.1038/ismej.2014.130 ISSN 1751-7362.
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Official URL: http://dx.doi.org/10.1038/ismej.2014.130
Abstract
Functionally important proteins at the interface of cell and soil are of potentially low abundance when compared with commonly recovered intracellular proteins. A novel approach was developed and used to extract the metaexoproteome, the subset of proteins found outside the cell, in the context of a soil enriched with the nitrogen-containing recalcitrant polymer chitin. The majority of proteins recovered was of bacterial origin and localized to the outer membrane or extracellular milieu. A wide variety of transporter proteins were identified, particularly those associated with amino-acid and phosphate uptake. The metaexoproteome extract retained chitinolytic activity and we were successful in detecting Nocardiopsis-like chitinases that correlated with the glycoside hydrolase family 18 (GH18) chi gene data and metataxonomic analysis. Nocardiopsis-like chitinases appeared to be solely responsible for chitinolytic activity in soil. This is the first study to detect and sequence bacterial exoenzymes with proven activity in the soil enzyme pool.
| Item Type: | Journal Article | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Subjects: | S Agriculture > S Agriculture (General) | ||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
| Library of Congress Subject Headings (LCSH): | Soil biochemistry, Extracellular enzymes | ||||||||
| Journal or Publication Title: | The ISME Journal | ||||||||
| Publisher: | Nature Publishing Group | ||||||||
| ISSN: | 1751-7362 | ||||||||
| Official Date: | 8 July 2014 | ||||||||
| Dates: |
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| Volume: | Volume 8 | ||||||||
| Number: | Number 10 | ||||||||
| Number of Pages: | 3 | ||||||||
| Page Range: | pp. 2148-2150 | ||||||||
| DOI: | 10.1038/ismej.2014.130 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||||
| Date of first compliant deposit: | 28 December 2015 | ||||||||
| Date of first compliant Open Access: | 28 December 2015 | ||||||||
| Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Seventh Framework Programme (European Commission) (FP7), Natural Environment Research Council (Great Britain) (NERC) | ||||||||
| Grant number: | KBBE-222625 (FP7, EU METAEXPLORE project (KBBE-222625)) |
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