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Optical properties of xanthene based fluorescent dyes studied by stretched-film linear dichroism
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Razmkhah, Kasra, Little, Haydn A., Sandhu, Sandeep, Dafforn, Tim and Rodger, Alison (2014) Optical properties of xanthene based fluorescent dyes studied by stretched-film linear dichroism. RSC Advances , Volume 4 (Number 71). pp. 37510-37515. doi:10.1039/c4ra06126h ISSN 2046-2069.
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Official URL: http://dx.doi.org/10.1039/C4RA06126H
Abstract
Xanthene dyes are commonly used to label proteins in order to probe their location and activity using fluorescence spectroscopy and microscopy. However, fundamental properties such as the polarizations of transitions for many of the dyes have not been available. In this paper we report the use of recently developed oxidised polyethylene (PEOX) stretched film linear dichroism to determine the transition polarizations of xanthene, 9-methyl-2,3,7-trihydroxy-6-fluorone, pyronin Y, pyronin B, fluorescein, and rhodamine 6G. The effect of the formation of higher order structures is also discussed when they occur. The dyes (except xanthene) all have an intense long-axis polarized transition in the region of 500 nm. They also have long-axis (//) polarized transitions from about 280 nm downwards in wavelength. There are suggestions of a weak short axis (⊥) polarized transition in the region of 320–350 nm in each case.
Item Type: | Journal Article | ||||||||
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Subjects: | Q Science > QP Physiology | ||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||||||
Library of Congress Subject Headings (LCSH): | Fluorescent probes, Fluorescence spectroscopy | ||||||||
Journal or Publication Title: | RSC Advances | ||||||||
Publisher: | Royal Society of Chemistry | ||||||||
ISSN: | 2046-2069 | ||||||||
Official Date: | 6 August 2014 | ||||||||
Dates: |
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Volume: | Volume 4 | ||||||||
Number: | Number 71 | ||||||||
Number of Pages: | 8 | ||||||||
Page Range: | pp. 37510-37515 | ||||||||
DOI: | 10.1039/c4ra06126h | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||
Date of first compliant deposit: | 28 December 2015 | ||||||||
Date of first compliant Open Access: | 28 December 2015 |
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