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The plasmodesmal protein PDLP1 localises to haustoria-associated membranes during downy mildew infection and regulates callose deposition
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Caillaud, Marie-Cécile, Wirthmueller, Lennart, Sklenar, Jan, Findlay, Kim, Piquerez, Sophie J. M., Jones, Alexandra M., Robatzek, Silke, Jones, Jonathan D. G. and Faulkner, Christine (2014) The plasmodesmal protein PDLP1 localises to haustoria-associated membranes during downy mildew infection and regulates callose deposition. PLoS Pathogens, Volume 10 (Number 11). Article number e1004496. doi:10.1371/journal.ppat.1004496 ISSN 1553-7374.
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Official URL: http://dx.doi.org/10.1371/journal.ppat.1004496
Abstract
The downy mildew pathogen Hyaloperonospora arabidopsidis (Hpa) is a filamentous oomycete that invades plant cells via sophisticated but poorly understood structures called haustoria. Haustoria are separated from the host cell cytoplasm and surrounded by an extrahaustorial membrane (EHM) of unknown origin. In some interactions, including Hpa-Arabidopsis, haustoria are progressively encased by host-derived, callose-rich materials but the molecular mechanisms by which callose accumulates around haustoria remain unclear. Here, we report that PLASMODESMATA-LOCATED PROTEIN 1 (PDLP1) is expressed at high levels in Hpa infected cells. Unlike other plasma membrane proteins, which are often excluded from the EHM, PDLP1 is located at the EHM in Hpa-infected cells prior to encasement. The transmembrane domain and cytoplasmic tail of PDLP1 are sufficient to convey this localization. PDLP1 also associates with the developing encasement but this association is lost when encasements are fully mature. We found that the pdlp1,2,3 triple mutant is more susceptible to Hpa while overexpression of PDLP1 enhances plant resistance, suggesting that PDLPs enhance basal immunity against Hpa. Haustorial encasements are depleted in callose in pdlp1,2,3 mutant plants whereas PDLP1 over-expression elevates callose deposition around haustoria and across the cell surface. These data indicate that PDLPs contribute to callose encasement of Hpa haustoria and suggests that the deposition of callose at haustoria may involve similar mechanisms to callose deposition at plasmodesmata.
Item Type: | Journal Article | ||||||||
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Subjects: | Q Science > QK Botany | ||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
Library of Congress Subject Headings (LCSH): | Oomycetes, Downy mildew diseases, Haustoria | ||||||||
Journal or Publication Title: | PLoS Pathogens | ||||||||
Publisher: | Public Library of Science | ||||||||
ISSN: | 1553-7374 | ||||||||
Official Date: | 13 November 2014 | ||||||||
Dates: |
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Volume: | Volume 10 | ||||||||
Number: | Number 11 | ||||||||
Article Number: | Article number e1004496 | ||||||||
DOI: | 10.1371/journal.ppat.1004496 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||
Date of first compliant deposit: | 28 December 2015 | ||||||||
Date of first compliant Open Access: | 28 December 2015 | ||||||||
Funder: | Gatsby Charitable Foundation (GCF), European Molecular Biology Organization (EMBO), Seventh Framework Programme (European Commission) (FP7), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Federation of European Biochemical Societies (FEBS) | ||||||||
Grant number: | ALTF 614-2009 (EMBO), FP7-PEOPLE-2009-IEF (FP7) |
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