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Dynamics of apelin receptor/G protein coupling in living cells
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Bai, Bo, Jiang, Yunlu, Cai, Xin and Chen, Jing (2014) Dynamics of apelin receptor/G protein coupling in living cells. Experimental Cell Research, Volume 328 (Number 2). pp. 401-409. doi:10.1016/j.yexcr.2014.08.035 ISSN 0014-4827.
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Official URL: http://dx.doi.org/10.1016/j.yexcr.2014.08.035
Abstract
During our research on apelin receptor (APJ) signalling in living cells with BRET and FRET, we demonstrated that apelin-13 stimulation can lead to the activation of Gαi2 or Gαi3 through undergoing a molecular rearrangement rather than dissociation in HEK293 cells expressing APJ. Furthermore, Gαo and Gαq also showed involvement in APJ activation through a classical dissociation model. However, both FRET signal and BRET ratio between fluorescent Gαi1 subunit and Gβγ subunits demonstrated little change after apelin-13 stimulation. These results demonstrated that stimulation of APJ with apelin-13 causes activation of Gαi2, Gαi3, Gαo, Gαq; among which Gαi2, Gαi3 were activated through a novel rearrangement process. These results provide helpful data for understanding APJ mediated G-protein signalling.
Item Type: | Journal Article | ||||||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Metabolic and Vascular Health (- until July 2016) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Journal or Publication Title: | Experimental Cell Research | ||||||||||
Publisher: | Academic Press | ||||||||||
ISSN: | 0014-4827 | ||||||||||
Official Date: | 1 November 2014 | ||||||||||
Dates: |
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Volume: | Volume 328 | ||||||||||
Number: | Number 2 | ||||||||||
Page Range: | pp. 401-409 | ||||||||||
DOI: | 10.1016/j.yexcr.2014.08.035 | ||||||||||
Status: | Peer Reviewed | ||||||||||
Publication Status: | Published | ||||||||||
Access rights to Published version: | Restricted or Subscription Access |
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