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Autoimmunity conferred by chs3-2D relies on CSA1, its adjacent TIR-NB-LRR encoding neighbour
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Xu, Fang, Zhu, Chipan, Cevik, Volkan, Johnson, Kaeli, Liu, Yanan, Sohn, Kee-Hoon, Jones, Jonathan D. G., Holub, E. B. and Li, Xin (2015) Autoimmunity conferred by chs3-2D relies on CSA1, its adjacent TIR-NB-LRR encoding neighbour. Scientific Reports, 5 . pp. 1-7. 8792. doi:10.1038/srep08792 ISSN 2045-2322.
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Official URL: http://dx.doi.org/10.1038/srep08792
Abstract
Plant innate immunity depends on the function of a large number of intracellular immune receptor proteins, the majority of which are structurally similar to mammalian nucleotidebinding oligomerization domain (NOD)-like receptor (NLR) proteins. CHILLING SENSITIVE 3 (CHS3) encodes an atypical Toll/Interleukin 1 Receptor (TIR)-type NLR protein with an additional Lin-11, Isl-1 and Mec-3 (LIM) domain at its C-terminus. The gain-of-function mutant allele chs3-2D exhibits severe dwarfism and constitutively activated defense responses, including enhanced resistance to virulent pathogens, high defence marker gene expression, and salicylic acid accumulation. To search for novel regulators involved in CHS3-mediated immune signaling, we conducted suppressor screens in the chs3-2D and chs3-2D pad4-1 genetic backgrounds. Alleles of sag101 and eds1-90 were isolated as complete suppressors of chs3-2D, and alleles of sgt1b were isolated as partial suppressors of chs3-2D pad4-1. These mutants suggest that SAG101, EDS1-90, and SGT1b are all positive regulators of CHS3-mediated defense signaling. Additionally, the TIR-type NLR-encoding CSA1 locus located genomically adjacent to CHS3 was found to be fully required for chs3-2D-mediated autoimmunity. CSA1 is located 3.9kb upstream of CHS3 and is transcribed in the opposite direction. Altogether, these data illustrate the distinct genetic requirements for CHS3-mediated defense signaling.
| Item Type: | Journal Article | ||||||||
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| Subjects: | Q Science > Q Science (General) Q Science > QK Botany S Agriculture > SB Plant culture |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
| Library of Congress Subject Headings (LCSH): | Plants -- Disease and pest resistance, Plants -- Genetics | ||||||||
| Journal or Publication Title: | Scientific Reports | ||||||||
| Publisher: | Nature Publishing Group | ||||||||
| ISSN: | 2045-2322 | ||||||||
| Official Date: | 5 March 2015 | ||||||||
| Dates: |
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| Volume: | 5 | ||||||||
| Number of Pages: | 7 | ||||||||
| Page Range: | pp. 1-7 | ||||||||
| Article Number: | 8792 | ||||||||
| DOI: | 10.1038/srep08792 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||||
| Date of first compliant deposit: | 28 July 2016 | ||||||||
| Date of first compliant Open Access: | 28 July 2016 | ||||||||
| Funder: | Natural Environment Research Council (Great Britain) (NERC) | ||||||||
| Grant number: | NE/H020691/1 |
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