Christie-Oleza, Joseph Alexander, Scanlan, David J. and Armengaud, Jean (2015) “You produce while I clean up”, a strategy revealed by exoproteomics during Synechococcus-Roseobacter interactions. Proteomics, 15 (20). pp. 3454-3462. doi:10.1002/pmic.201400562 ISSN 1615-9853.

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Abstract

Most of the energy that is introduced into the oceans by photosynthetic primary producers is in the form of organic matter that then sustains the rest of the food web, from micro to macro-organisms. However, it is the interactions between phototrophs and heterotrophs that are vital to maintaining the nutrient balance of marine microbiomes that ultimately feed these higher trophic levels. The primary produced organic matter is mostly re-mineralised by heterotrophic microorganisms but, because most of the oceanic dissolved organic matter (DOM) is in the form of biopolymers, and microbial membrane transport systems operate with molecules <0.6 kDa, it must be hydrolysed outside the cell before a microorganism can acquire it. As a simili of the marine microbiome, we analysed, using state-of-the-art proteomics, the exoproteomes obtained from synthetic communities combining specific Roseobacter (Ruegeria pomeroyi DSS-3, Roseobacter denitrificans OCh114 and Dinoroseobacter shibae DFL-12) and Synechococcus strains (WH7803 and WH8102). This approach identified the repertoire of hydrolytic enzymes secreted by Roseobacter, opening up the black box of heterotrophic transformation/re-mineralisation of biopolymers generated by marine phytoplankton. As well as highlighting interesting exoenzymes this strategy also allowed us to infer clues on the molecular basis of niche partitioning.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology
Divisions:	Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH):	Photosynthetic bacteria, Marine microbiology
Journal or Publication Title:	Proteomics
Publisher:	Wiley-Blackwell Publishing Ltd.
ISSN:	1615-9853
Official Date:	15 October 2015
Dates:	Date Event 15 October 2015 Published 27 February 2015 Available 24 February 2015 Accepted 30 November 2014 Submitted
Volume:	15
Number:	20
Number of Pages:	9
Page Range:	pp. 3454-3462
DOI:	10.1002/pmic.201400562
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Open Access (Creative Commons)
Date of first compliant deposit:	28 July 2016
Date of first compliant Open Access:	28 July 2016
Funder:	Seventh Framework Programme (European Commission) (FP7), Marie Skłodowska-Curie actions, Natural Environment Research Council (Great Britain) (NERC), France. Commissariat à l'énergie atomique (CEA)
Grant number:	PIEF-GA-2010-272593 (FP7), NE/K009044/1 (NERC)

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