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Genomic and proteomic characterization of two novel siphovirus infecting the sedentary facultative epibiont cyanobacterium Acaryochloris marina
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Chan, Yi-Wah, Millard, Andrew D., Wheatley, Peter J., Holmes, Antony B., Mohr, Remus, Whitworth, Anna L., Mann, Nicholas H., Larkum, Anthony W. D., Hess, Wolfgang R., Scanlan, David J. and Clokie, Martha R. J. (2015) Genomic and proteomic characterization of two novel siphovirus infecting the sedentary facultative epibiont cyanobacterium Acaryochloris marina. Environmental Microbiology, 17 (11). pp. 4239-4252. doi:10.1111/1462-2920.12735 ISSN 1462-2912.
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Official URL: http://dx.doi.org/10.1111/1462-2920.12735
Abstract
Acaryochloris marina is a symbiotic species of cyanobacteria that is capable of utilizing far-red light. We report the characterization of the phages A-HIS1 and A-HIS2, capable of infecting Acaryochloris. Morphological characterization of these phages places them in the family Siphoviridae. However, molecular characterization reveals that they do not show genetic similarity with any known siphoviruses. While the phages do show synteny between each other, the nucleotide identity between the phages is low at 45–67%, suggesting they diverged from each other some time ago. The greatest number of genes shared with another phage (a myovirus infecting marine Synechococcus) was four. Unlike most other cyanophages and in common with the Siphoviridae infecting Synechococcus, no photosynthesis-related genes were found in the genome. CRISPR (clustered regularly interspaced short palindromic repeats) spacers from the host Acaryochloris had partial matches to sequences found within the phages, which is the first time CRISPRs have been reported in a cyanobacterial/cyanophage system. The phages also encode a homologue of the proteobacterial RNase T. The potential function of RNase T in the mark-up or digestion of crRNA hints at a novel mechanism for evading the host CRISPR system.
Item Type: | Journal Article | ||||||||||
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Subjects: | Q Science > QR Microbiology | ||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) Faculty of Science, Engineering and Medicine > Science > Physics Faculty of Science, Engineering and Medicine > Research Centres > Warwick Systems Biology Centre |
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Library of Congress Subject Headings (LCSH): | Cyanobacteria, Bacteriophages, Prokaryotes, Ribonucleases, Viruses | ||||||||||
Journal or Publication Title: | Environmental Microbiology | ||||||||||
Publisher: | Wiley-Blackwell Publishing Ltd. | ||||||||||
ISSN: | 1462-2912 | ||||||||||
Official Date: | November 2015 | ||||||||||
Dates: |
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Volume: | 17 | ||||||||||
Number: | 11 | ||||||||||
Page Range: | pp. 4239-4252 | ||||||||||
DOI: | 10.1111/1462-2920.12735 | ||||||||||
Status: | Peer Reviewed | ||||||||||
Publication Status: | Published | ||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||
Date of first compliant deposit: | 16 December 2016 | ||||||||||
Date of first compliant Open Access: | 16 December 2016 | ||||||||||
Funder: | Natural Environment Research Council (Great Britain) (NERC), University of Warwick. Molecular Organisation and Assembly in Cells, Engineering and Physical Sciences Research Council (EPSRC), Deutscher Akademischer Austauschdienst (DAAD), Deutsche Forschungsgemeinschaft (DFG) | ||||||||||
Grant number: | NE/E01089X/1 (NERC), HE2544/8-1 (DAAD) | ||||||||||
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