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Efficient export of human growth hormone, interferon α2b and antibody fragments to the periplasm by the Escherichia coli Tat pathway in the absence of prior disulfide bond formation
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Alanen, Heli I., Walker, Kelly L., Lourdes Velez Suberbie, M., Matos, Cristina F.R.O., Bönisch, Sarah, Freedman, Robert B., Keshavarz-Moore, Eli, Ruddock, Lloyd W. and Robinson, Colin (2015) Efficient export of human growth hormone, interferon α2b and antibody fragments to the periplasm by the Escherichia coli Tat pathway in the absence of prior disulfide bond formation. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1853 (Number 3). pp. 756-763. doi:10.1016/j.bbamcr.2014.12.027 ISSN 0167-4889.
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Official URL: http://dx.doi.org/10.1016/j.bbamcr.2014.12.027
Abstract
Numerous therapeutic proteins are expressed in Escherichia coli and targeted to the periplasm in order to facilitate purification and enable disulfide bond formation. Export is normally achieved by the Sec pathway, which transports proteins through the plasma membrane in a reduced, unfolded state. The Tat pathway is a promising alternative means of export, because it preferentially exports correctly folded proteins; however, the reducing cytoplasm of standard strains has been predicted to preclude export by Tat of proteins that contain disulfide bonds in the native state because, in the reduced state, they are sensed as misfolded and rejected. Here, we have tested a series of disulfide-bond containing biopharmaceuticals for export by the Tat pathway in CyDisCo strains that do enable disulfide bond formation in the cytoplasm. We show that interferon α2b, human growth hormone (hGH) and two antibody fragments are exported with high efficiency; surprisingly, however, they are efficiently exported even in the absence of cytoplasmic disulfide formation. The exported proteins acquire disulfide bonds in the periplasm, indicating that the normal disulfide oxidation machinery is able to act on the proteins. Tat-dependent export of hGH proceeds even when the disulfide bonds are removed by substitution of the Cys residues involved, suggesting that these substrates adopt tertiary structures that are accepted as fully-folded by the Tat machinery.
Item Type: | Journal Article | ||||||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||
Journal or Publication Title: | Biochimica et Biophysica Acta (BBA) - Molecular Cell Research | ||||||||||
Publisher: | Elsevier | ||||||||||
ISSN: | 0167-4889 | ||||||||||
Official Date: | March 2015 | ||||||||||
Dates: |
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Volume: | Volume 1853 | ||||||||||
Number: | Number 3 | ||||||||||
Page Range: | pp. 756-763 | ||||||||||
DOI: | 10.1016/j.bbamcr.2014.12.027 | ||||||||||
Status: | Peer Reviewed | ||||||||||
Publication Status: | Published | ||||||||||
Access rights to Published version: | Restricted or Subscription Access |
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