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A novel collagen gel-based measurement technique for quantitation of cell contraction force
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Jin, Tianrong, Li, L., Siow, R. C. M. and Liu, Kuo-Kang (2015) A novel collagen gel-based measurement technique for quantitation of cell contraction force. Journal of The Royal Society Interface, Volume 12 (Number 106). Article number 20141365. doi:10.1098/rsif.2014.1365 ISSN 1742-5689.
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Official URL: http://dx.doi.org/10.1098/rsif.2014.1365
Abstract
Cell contraction force plays an important role in wound healing, inflammation, angiogenesis and metastasis. This study describes a novel method to quantify single cell contraction force in vitro using human aortic adventitial fibroblasts embedded in a collagen gel. The technique is based on a depth sensing nano-indentation tester to measure the thickness and elasticity of collagen gels containing stimulated fibroblasts and a microscopy imaging system to estimate the gel area. In parallel, a simple theoretical model has been developed to calculate cell contraction force based on the measured parameters. Histamine (100 µM) was used to stimulate fibroblast contraction while the myosin light chain kinase inhibitor ML-7 (25 µM) was used to inhibit cell contraction. The collagen matrix used in the model provides a physiological environment for fibroblast contraction studies. Measurement of changes in collagen gel elasticity and thickness arising from histamine treatments provides a novel convenient technique to measure cell contraction force within a collagen matrix. This study demonstrates that histamine can elicit a significant increase in contraction force of fibroblasts embedded in collagen, while the Young's modulus of the gel decreases due to the gel degradation.
| Item Type: | Journal Article | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Subjects: | Q Science > QH Natural history > QH301 Biology | ||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Engineering > Engineering | ||||||||
| Library of Congress Subject Headings (LCSH): | Cytology—Research | ||||||||
| Journal or Publication Title: | Journal of The Royal Society Interface | ||||||||
| Publisher: | The Royal Society Publishing | ||||||||
| ISSN: | 1742-5689 | ||||||||
| Official Date: | 8 April 2015 | ||||||||
| Dates: |
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| Volume: | Volume 12 | ||||||||
| Number: | Number 106 | ||||||||
| Article Number: | Article number 20141365 | ||||||||
| DOI: | 10.1098/rsif.2014.1365 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||||
| Date of first compliant deposit: | 29 December 2015 | ||||||||
| Date of first compliant Open Access: | 27 April 2016 | ||||||||
| Funder: | Leverhulme Trust (LT), British Heart Foundation | ||||||||
| Grant number: | PRG-2012-738; FS/09/037/27827; |
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