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XoxFencoding an alternative methanol dehydrogenase is widespread in coastal marine environments
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Taubert, Martin, Grob, Carolina, Howat, Alexandra M., Burns, Oliver J., Dixon, Joanna L., Chen, Yin and Murrell, J. C. (J. Colin) (2015) XoxFencoding an alternative methanol dehydrogenase is widespread in coastal marine environments. Environmental Microbiology, 17 (10). pp. 3937-3948. doi:10.1111/1462-2920.12896 ISSN 1462-2912.
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WRAP_0676431-lf-050615-20150417_draft_1.pdf - Accepted Version - Requires a PDF viewer. Download (963Kb) | Preview |
Official URL: http://dx.doi.org/10.1111/1462-2920.12896
Abstract
The xoxF gene, encoding a pyrroloquinoline quinone-dependent methanol dehydrogenase, is found in all known proteobacterial methylotrophs. In several newly discovered methylotrophs, XoxF is the active methanol dehydrogenase, catalysing the oxidation of methanol to formaldehyde. Apart from that, its potential role in methylotrophy and carbon cycling is unknown. So far, the diversity of xoxF in the environment has received little attention. We designed PCR primer sets targeting clades of the xoxF gene, and used 454 pyrosequencing of PCR amplicons obtained from DNA of four coastal marine environments for a unique assessment of the diversity of xoxF in these habitats. Phylogenetic analysis of the data obtained revealed a high diversity of xoxF genes from two of the investigated clades, and substantial differences in sequence composition between environments. Sequences were classified as being related to a wide range of both methylotrophs and non-methylotrophs from Alpha-, Beta- and Gammaproteobacteria. The most prominent sequences detected were related to the family Rhodobacteraceae, the genus Methylotenera and the OM43 clade of Methylophilales, and are thus related to organisms that employ XoxF for methanol oxidation. Furthermore, our analyses revealed a high degree of so far undescribed sequences, suggesting a high number of unknown species in these habitats.
| Item Type: | Journal Article | ||||||||
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| Subjects: | Q Science > QD Chemistry Q Science > QH Natural history Q Science > QP Physiology Q Science > QR Microbiology S Agriculture > SH Aquaculture. Fisheries. Angling |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
| Library of Congress Subject Headings (LCSH): | Methylobacterium, Biochemistry, Methylotrophic microorganisms , Marine ecology , Marine microbial ecology | ||||||||
| Journal or Publication Title: | Environmental Microbiology | ||||||||
| Publisher: | Blackwell | ||||||||
| ISSN: | 1462-2912 | ||||||||
| Official Date: | 22 October 2015 | ||||||||
| Dates: |
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| Volume: | 17 | ||||||||
| Number: | 10 | ||||||||
| Page Range: | pp. 3937-3948 | ||||||||
| DOI: | 10.1111/1462-2920.12896 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||||
| Date of first compliant deposit: | 29 December 2015 | ||||||||
| Date of first compliant Open Access: | 4 May 2016 | ||||||||
| Funder: | Gordon and Betty Moore Foundation (GBMF), Earth & Life Systems Alliance (ELSA) | ||||||||
| Grant number: | GBMF3303 (GBMF) |
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