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Nanomechanical investigation of soft biological cell adhesion using atomic force microscopy
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Siamantouras, Eleftherios, Hills, Claire E., Squires, Paul E. and Liu, Kuo-Kang (2015) Nanomechanical investigation of soft biological cell adhesion using atomic force microscopy. Cellular and Molecular Bioengineering, Volume 8 (Number 1). pp. 22-31. doi:10.1007/s12195-014-0359-2 ISSN 1865-5025.
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WRAP_0874042-es-090615-aam_of_cellularmolecularbioeng_liu_et_al.pdf - Accepted Version - Requires a PDF viewer. Download (864Kb) | Preview |
Official URL: http://dx.doi.org/10.1007/s12195-014-0359-2
Abstract
Mechanical coupling between living cells is a complex process that is important for a variety of biological processes. In this study the effects of specific biochemical treatment on cell-to-cell adhesion and single cell mechanics were systematically investigated using atomic force microscopy (AFM) single cell force spectroscopy. Functionalised AFM tipless cantilevers were used for attaching single suspended cells that were brought in contact with substrate cells. Cell-to-cell adhesion parameters, such as maximum unbinding force (F max) and work or energy of detachment (W D), were extracted from the retraction force–displacement (F–d) curves. AFM indentation experiments were performed by indenting single cells with a spherical microbead attached to the cantilever. Hertzian contact model was applied to determine the elastic modulus (E) of single cells. Following treatment of the cells with neutralising antibody for epithelial (E)-cadherin, F max was increased by 25%, whereas W D decreased by 11% in response to a 43% increase in E. The results suggest that although the adhesion force between cells was increased after treatment, the energy of adhesion was decreased due to the reduced displacement separation as manifested by the loss of elastic deformation. Conclusively, changes in single cell mechanics are important underlying factors contributing to cell-to-cell adhesion and hence cytomechanical characterization is critical for cell adhesion measurements.
Item Type: | Journal Article | ||||||||||
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Subjects: | Q Science > QH Natural history > QH301 Biology | ||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Engineering > Engineering Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) |
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Library of Congress Subject Headings (LCSH): | Cytology -- Research, Cells -- Mechanical properties | ||||||||||
Journal or Publication Title: | Cellular and Molecular Bioengineering | ||||||||||
Publisher: | Springer New York LLC | ||||||||||
ISSN: | 1865-5025 | ||||||||||
Official Date: | March 2015 | ||||||||||
Dates: |
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Volume: | Volume 8 | ||||||||||
Number: | Number 1 | ||||||||||
Page Range: | pp. 22-31 | ||||||||||
DOI: | 10.1007/s12195-014-0359-2 | ||||||||||
Status: | Peer Reviewed | ||||||||||
Publication Status: | Published | ||||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||||
Date of first compliant deposit: | 29 December 2015 | ||||||||||
Date of first compliant Open Access: | 29 December 2015 | ||||||||||
Funder: | British Diabetic Association, Leverhulme Trust (LT) | ||||||||||
Grant number: | BDA: 09/0003913 and 12/0004546; PRG-2012-738; |
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