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Differential role for transcription factor oct 4 nucleocytoplasmic dynamics in somatic cell reprogramming and self-renewal of embryonic stem cells
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Oka, Masahiro, Moriyama, Tetsuji, Asally, Munehiro, Kawakami, Koichi and Yoneda, Yoshihiro (2013) Differential role for transcription factor oct 4 nucleocytoplasmic dynamics in somatic cell reprogramming and self-renewal of embryonic stem cells. Journal of Biological Chemistry, 288 (21). pp. 15085-15097. doi:10.1074/jbc.M112.448837 ISSN 0021-9258.
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Official URL: http://dx.doi.org/10.1074/jbc.M112.448837
Abstract
Oct4 is a member of the POU family of transcription factors and plays a critical role in both maintenance of the undifferentiated state of embryonic stem (ES) cells and in the reprogramming of somatic cells to induced pluripotent stem cells. Oct4 is imported into the nucleus where it functions as a transcription factor; however, the spatiotemporal dynamic behavior of Oct4 remains largely unknown. In the present study we show that Oct4 is a nucleocytoplasmic shuttling protein. Furthermore, although Oct4 mutants with altered nuclear import/export activity were able to maintain the self-renewal of ES cells, they displayed limited potential for cellular reprogramming. These results indicate that the intracellular localization of Oct4, which is dependent on nucleocytoplasmic shuttling, must be more strictly regulated for cellular reprogramming, suggesting that Oct4 plays differential roles in the self-renewal of ES cells and in somatic cell reprogramming.
| Item Type: | Journal Article | ||||||||
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| Subjects: | Q Science > QH Natural history | ||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Biological Sciences ( -2010) Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) |
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| Library of Congress Subject Headings (LCSH): | Embryonic stem cells -- Research | ||||||||
| Journal or Publication Title: | Journal of Biological Chemistry | ||||||||
| Publisher: | American Society for Biochemistry and Molecular Biology | ||||||||
| ISSN: | 0021-9258 | ||||||||
| Official Date: | 24 May 2013 | ||||||||
| Dates: |
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| Volume: | 288 | ||||||||
| Number: | 21 | ||||||||
| Number of Pages: | 9 | ||||||||
| Page Range: | pp. 15085-15097 | ||||||||
| DOI: | 10.1074/jbc.M112.448837 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||||
| Funder: | Japan. Monbu Kagakushō [Japan. Ministry of Education, Culture, Sports, Science and Technology] (MK), Kagaku Gijutsu Shinkō Kikō [Japan Science and Technology Agency] (JST) |
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