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Standards for plant synthetic biology : a common syntax for exchange of DNA parts
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(2015) Standards for plant synthetic biology : a common syntax for exchange of DNA parts. New Phytologist, 208 (1). pp. 13-19. doi:10.1111/nph.13532 ISSN 0028-646X.
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Official URL: http://dx.doi.org/10.1111/nph.13532
Abstract
Inventors in the field of mechanical and electronic engineering can access multitudes of components and, thanks to standardization, parts from different manufacturers can be used in combination with each other. The introduction of BioBrick standards for the assembly of characterized DNA sequences was a landmark in microbial engineering, shaping the field of synthetic biology. Here, we describe a standard for Type IIS restriction endonuclease-mediated assembly, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units. This standard has been developed and agreed by representatives and leaders of the international plant science and synthetic biology communities, including inventors, developers and adopters of Type IIS cloning methods. Our vision is of an extensive catalogue of standardized, characterized DNA parts that will accelerate plant bioengineering.
| Item Type: | Journal Article | ||||||
|---|---|---|---|---|---|---|---|
| Subjects: | Q Science > QH Natural history | ||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
| Library of Congress Subject Headings (LCSH): | Clones (Plants), Synthetic biology | ||||||
| Journal or Publication Title: | New Phytologist | ||||||
| Publisher: | Wiley-Blackwell Publishing Ltd. | ||||||
| ISSN: | 0028-646X | ||||||
| Official Date: | October 2015 | ||||||
| Dates: |
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| Volume: | 208 | ||||||
| Number: | 1 | ||||||
| Page Range: | pp. 13-19 | ||||||
| DOI: | 10.1111/nph.13532 | ||||||
| Status: | Peer Reviewed | ||||||
| Publication Status: | Published | ||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||
| Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Spain. Ministerio de Economía y Competitividad [Ministry of Economy and Competitiveness] (MINECO), John Innes Centre, Gatsby Charitable Foundation (GCF), Bill & Melinda Gates Foundation, United States. Department of Energy, COST Action | ||||||
| Grant number: | BB/K005952/1 (BBSRC), BB/L02182X/1 (BBSRC), BB/L014130/1 (BBSRC), BIO2013-42193-R (MINECO) DE-AC02-05CH1123 (DOE), FA1006 (COST) |
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