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Dynamic changes in lipid droplet-associated proteins in the “browning” of white adipose tissues
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Barneda, David, Frontini, Andrea, Cinti, Saverio and Christian, Mark (2013) Dynamic changes in lipid droplet-associated proteins in the “browning” of white adipose tissues. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1831 (5). pp. 924-933. doi:10.1016/j.bbalip.2013.01.015 ISSN 0167-4889.
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Official URL: http://dx.doi.org/10.1016/j.bbalip.2013.01.015
Abstract
The morphological and functional differences between lipid droplets (LDs) in brown (BAT) and white (WAT) adipose tissues will largely be determined by their associated proteins. Analysing mRNA expression in mice fat depots we have found that most LD protein genes are expressed at higher levels in BAT, with the greatest differences observed for Cidea and Plin5. Prolonged cold exposure, which induces the appearance of brown-like adipocytes in mice WAT depots, was accompanied with the potentiation of the lipolytic machinery, with changes in ATGL, CGI-58 and G0S2 gene expression. However the major change detected in WAT was the enhancement of Cidea mRNA. Together with the increase in Cidec, it indicates that LD enlargement through LD-LD transference of fat is an important process during WAT browning. To study the dynamics of this phenotypic change, we have applied 4D confocal microscopy in differentiated 3T3-L1 cells under sustained β-adrenergic stimulation. Under these conditions the cells experienced a LD remodelling cycle, with progressive reduction on the LD size by lipolysis, followed by the formation of new LDs, which were subjected to an enlargement process, likely to be CIDE-triggered, until the cell returned to the basal state. This transformation would be triggered by the activation of a thermogenic futile cycle of lipolysis/lipogenesis and could facilitate the molecular mechanism for the unilocular to multilocular transformation during WAT browning. This article is part of a Special Issue entitled Brown and White Fat: From Signaling to Disease.
| Item Type: | Journal Article | ||||||||||||
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| Subjects: | Q Science > QP Physiology | ||||||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Metabolic and Vascular Health (- until July 2016) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Adipose tissues, Brown adipose tissue, Lipolysis, Messenger RNA | ||||||||||||
| Journal or Publication Title: | Biochimica et Biophysica Acta (BBA) - Molecular Cell Research | ||||||||||||
| Publisher: | Elsevier | ||||||||||||
| ISSN: | 0167-4889 | ||||||||||||
| Official Date: | May 2013 | ||||||||||||
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| Volume: | 1831 | ||||||||||||
| Number: | 5 | ||||||||||||
| Page Range: | pp. 924-933 | ||||||||||||
| DOI: | 10.1016/j.bbalip.2013.01.015 | ||||||||||||
| Status: | Peer Reviewed | ||||||||||||
| Publication Status: | Published | ||||||||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||||||||
| RIOXX Funder/Project Grant: |
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