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Protective actions of globular and full-length adiponectin on human endothelial cells : novel insights into adiponectin-induced angiogenesis
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Adya, Raghu, Tan, Bee K., Chen, Jing and Randeva, Harpal S. (2012) Protective actions of globular and full-length adiponectin on human endothelial cells : novel insights into adiponectin-induced angiogenesis. Journal of Vascular Research, 49 (6). pp. 534-543. 338279. doi:10.1159/000338279 ISSN 1018-1172.
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Official URL: http://dx.doi.org/10.1159/000338279
Abstract
Background/aims:
Adiponectin levels are decreased in diabetes and atherosclerosis. Coexisting hyperglycaemia and systemic inflammation predisposes to dysregulated angiogenesis and vascular disease. We investigated the effect of globular adiponectin (gAd) and full-length adiponectin (fAd) on angiogenesis and pro-angiogenic molecules, i.e. matrix metalloproteinase (MMP)-2, MMP-9 and vascular endothelial growth factor (VEGF), in human microvascular endothelial cells (HMEC-1).
Methods:
Angiogenesis was assessed by studying capillary tube formation in HMEC-1 on growth factor-reduced Matrigel. Endothelial cell migration assay was performed in a modified Boyden chamber.
Results:
Endothelial cell proliferation, in vitro migration and angiogenesis were significantly increased by gAd (mediated by AdipoR1, AMPK-Akt pathways), and gAd significantly increased MMP-2, MMP-9 and VEGF expression levels. The effect of gAd on VEGF appears to be mediated by AdipoR1, whilst the effect of gAd on MMP-2 and MMP-9 appears to be mediated by AdipoR1 and AdipoR2. Only endothelial cell proliferation was significantly increased by fAd in human microvascular endothelial cells and appears to be mediated by AdipoR2. No significant effects on MMP-2, MMP-9 and VEGF were observed. Importantly, gAd decreased glucose and C-reactive protein-induced angiogenesis with a concomitant reduction in MMP-2, MMP-9 and VEGF in HMEC-1 cells.
Conclusion:
We report novel insights into the mechanisms of adiponectin on angiogenesis.
| Item Type: | Journal Article | ||||||||||
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| Subjects: | Q Science > QP Physiology | ||||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Metabolic and Vascular Health (- until July 2016) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Reproductive Health ( - until July 2016) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Endothelial cells, Neovascularization | ||||||||||
| Journal or Publication Title: | Journal of Vascular Research | ||||||||||
| Publisher: | Karger | ||||||||||
| ISSN: | 1018-1172 | ||||||||||
| Official Date: | October 2012 | ||||||||||
| Dates: |
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| Volume: | 49 | ||||||||||
| Number: | 6 | ||||||||||
| Number of Pages: | 10 | ||||||||||
| Page Range: | pp. 534-543 | ||||||||||
| Article Number: | 338279 | ||||||||||
| DOI: | 10.1159/000338279 | ||||||||||
| Status: | Peer Reviewed | ||||||||||
| Publication Status: | Published | ||||||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||||||
| Funder: | General Charities of the City of Coventry (GCCC) |
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