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The phosphoenolpyruvate : sugar phosphotransferase system is involved in sensitivity to the glucosylated bacteriocin sublancin
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Garcia De Gonzalo, C. V., Denham, Emma, Mars, Ruben A. T., Stülke, J., Donk, W. A. van der and Dijl, Jan Maarten van (2015) The phosphoenolpyruvate : sugar phosphotransferase system is involved in sensitivity to the glucosylated bacteriocin sublancin. Antimicrobial agents and chemotherapy, 59 (11). pp. 6844-6854. doi:10.1128/AAC.01519-15 ISSN 0066-4804.
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WRAP_9577424-ms-180815-antimicrob._agents_chemother.-2015-garcia_de_gonzalo-aac.01519-15.pdf - Accepted Version - Requires a PDF viewer. Download (1103Kb) | Preview |
Official URL: http://dx.doi.org/10.1128/AAC.01519-15
Abstract
The mode of action of a group of glycosylated antimicrobial peptides known as glycocins remains to be elucidated. In the current study on one glycocin, sublancin, we identified the phosphoenolpyruvate:sugar phosphotransferase system (PTS) of Bacillus species as a key player in bacterial sensitivity. Sublancin kills several Gram-positive bacteria such as Bacillus species and Staphylococcus aureus, including methicillin-resistant S. aureus (MRSA). Unlike other classes of bacteriocins for which the PTS is involved in their mechanism of action, we show that the addition of PTS-requiring sugars leads to increased resistance rather than an increased sensitivity, suggesting that sublancin has a distinct mechanism of action. Collectively, our present mutagenesis and genomic studies demonstrate that, in particular, the histidine-containing phosphocarrier protein (HPr) and domain A of enzyme II (PtsG) are critical determinants for the bacterial sensitivity to sublancin.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QR Microbiology | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Library of Congress Subject Headings (LCSH): | Bacteriocins, Phosphotransferases, Pyruvate kinase | ||||
Journal or Publication Title: | Antimicrobial agents and chemotherapy | ||||
Publisher: | American Society for Microbiology | ||||
ISSN: | 0066-4804 | ||||
Official Date: | 17 August 2015 | ||||
Dates: |
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Volume: | 59 | ||||
Number: | 11 | ||||
Page Range: | pp. 6844-6854 | ||||
DOI: | 10.1128/AAC.01519-15 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Date of first compliant deposit: | 30 December 2015 | ||||
Date of first compliant Open Access: | 30 December 2015 | ||||
Funder: | European Commission (EC), Systems Biology of Microorganisms (SysMO), National Institute of General Medical Sciences (U.S.) (NIGMS), National Institutes of Health (U.S.) (NIH), Nederlandse Organisatie voor Wetenschappelijk Onderzoek [Netherlands Organisation for Scientific Research] (NWO) | ||||
Grant number: | LSHG-CT-2006-037469 (EC), 244093 (EC), BACELL SysMO2 (SysMO), 5T32-GM070421 (NIGMS) (NIH), |
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