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Mining for nonribosomal peptide synthetase and polyketide synthase genes revealed a high level of diversity in the sphagnum bog metagenome
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Müller, Christina A., Oberauner-Wappis, Lisa, Peyman, Armin, Amos, Gregory C. A., Wellington, E. M. H. and Berg, Gabriele (2015) Mining for nonribosomal peptide synthetase and polyketide synthase genes revealed a high level of diversity in the sphagnum bog metagenome. Applied and Environmental Microbiology, 81 (15). pp. 5064-5072. doi:10.1128/AEM.00631-15 ISSN 0099-2240.
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Official URL: http://dx.doi.org/10.1128/AEM.00631-15
Abstract
Sphagnum bog ecosystems are one of the oldest vegetation forms harbouring a specific microbial community, which is known to produce an exceptionally wide variety of bioactive substances. Although the Sphagnum metagenome indicate a rich secondary metabolism, the genes are not yet explored. To analyse non-ribosomal peptide synthetases (NRPS) and polyketide synthases (PKS) the diversity of NRPS and PKS genes in Sphagnum-associated metagenome was investigated by in silico data mining and sequence-based screening (PCR-amplification of 9500 fosmid clones). The in silico Illumina-based metagenomic approach resulted in the identification of 279 NRPS, 346 PKS, as well as 40 PKS-NRPS hybrid gene sequences. Occurrence of NRPS sequences was strongly dominated by the phyla Protebacteria, especially by the genus Burkholderia, while PKS sequences were mainly affiliated to Actinobacteria. Thirteen novel NRPS-related sequences were identified by PCR-amplification screening, displaying amino acid sequence identities of 48 to 91% to annotated sequences of the phyla Proteobacteria, Actinobacteria and Cyanobacteria. Some of the identified metagenomic clones showed closest similarity to peptide synthases from Burkholderia or Lysobacter, which are emerging bacterial sources of yet undescribed bioactive metabolites. This study highlights the role of the extreme natural ecosystems as a promising source for detection of secondary compounds and enzymes, serving as a source for biotechnological applications.
Item Type: | Journal Article | ||||||||
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Subjects: | Q Science > QK Botany | ||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
Library of Congress Subject Headings (LCSH): | Peat mosses, Metagenomics, Peptides -- Synthesis | ||||||||
Journal or Publication Title: | Applied and Environmental Microbiology | ||||||||
Publisher: | American Society for Microbiology | ||||||||
ISSN: | 0099-2240 | ||||||||
Official Date: | August 2015 | ||||||||
Dates: |
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Volume: | 81 | ||||||||
Number: | 15 | ||||||||
Number of Pages: | 9 | ||||||||
Page Range: | pp. 5064-5072 | ||||||||
DOI: | 10.1128/AEM.00631-15 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||
Date of first compliant deposit: | 31 December 2015 | ||||||||
Date of first compliant Open Access: | 29 February 2016 | ||||||||
Funder: | Austria. Bundesministerium für Wirtschaft, Familie und Jugend [Federal Ministry of Economy, Family, and Youth] (BMWFJ), Austria. Bundesministerium für Verkehr, Innovation und Technologie [Federal Ministry of Transport, Innovation and Technology ] (BMVIT), Styria (Austria), Standortagentur Tirol, Österreichische Forschungsförderungsgesellschaft [Austrian Research Promotion Agency], Zentrum für Innovation und Technologie (Vienna) (ZIT) |
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