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SADB phosphorylation of γ-tubulin regulates centrosome duplication
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Alvarado-Kristensson, María, Rodríguez, María Josefa, Silio, Virginia, Valpuesta, José M. and Carrera, Ana C. (2009) SADB phosphorylation of γ-tubulin regulates centrosome duplication. Nature Cell Biology, 11 (9). pp. 1081-1092. doi:10.1038/ncb1921 ISSN 1465-7392.
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Official URL: http://dx.doi.org/10.1038/ncb1921
Abstract
Symmetrical cell division requires duplication of DNA and protein content to generate two daughter cells. Centrosomes also duplicate during cell division, but the mechanism controlling this process is incompletely understood. We describe an alternative splice form of SadB encoding a short SADB Ser/Thr kinase whose activity fluctuates during the cell cycle, localizes to centrosomes, and controls centrosome duplication. Reduction of endogenous SADB levels diminished centrosome numbers, whereas enhanced SADB expression induced centrosome amplification. SADB exerted this action through phosphorylation of gamma-tubulin on Ser 131, as expression of a phosphomimetic Ser 131-to-Asp gamma-tubulin mutant alone increased centrosome numbers, whereas non-phosphorylatable Ala 131-gamma-tubulin impaired centrosome duplication. We propose that SADB kinase activity controls centrosome homeostasis by regulating phosphorylation of gamma-tubulin.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Journal or Publication Title: | Nature Cell Biology | ||||
Publisher: | Nature Publishing Group | ||||
ISSN: | 1465-7392 | ||||
Official Date: | 2009 | ||||
Dates: |
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Volume: | 11 | ||||
Number: | 9 | ||||
Page Range: | pp. 1081-1092 | ||||
DOI: | 10.1038/ncb1921 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
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