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Revised morning loops of the Arabidopsis circadian clock based on analyses of direct regulatory interactions
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Adams, Sally, Manfield, Ian, Stockley, Peter and Carré, Isabelle A. (2015) Revised morning loops of the Arabidopsis circadian clock based on analyses of direct regulatory interactions. PLoS One, 10 (12). e0143943. doi:10.1371/journal.pone.0143943 ISSN 1932-6203.
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Official URL: http://dx.doi.org/10.1371/journal.pone.0143943
Abstract
The network structure of the plant circadian clock is complex and direct regulatory interactions between individual components have proven particularly difficult to predict from genetic analyses. Here, we systematically investigate in vivo binding interactions between the morning-specific transcription factor, LATE ELONGATED HYPOCOTYL (LHY) and the promoters of other components of the network. We then demonstrate the functionality of these interactions by testing the responsiveness of the target gene to an ethanol-induced change in expression level of the LHY protein. We uncover novel, negative autoregulatory feedback loops from LHY and the closely related CIRCADIAN CLOCK ASSOCIATED-1 (CCA1) onto their own and each other’s expression. Furthermore we show that LHY acts as a repressor of all other clock components, including PSEUDO-RESPONSE REGULATORs (PRRs) 9 and 7, which were previously thought to be positive regulatory targets. These experimental results lead to a substantial revision of the morning loops of the clock.
Item Type: | Journal Article | ||||||||
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Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QK Botany |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
Library of Congress Subject Headings (LCSH): | Circadian rhythms, Plant proteins, Plant genetics | ||||||||
Journal or Publication Title: | PLoS One | ||||||||
Publisher: | Public Library of Science | ||||||||
ISSN: | 1932-6203 | ||||||||
Official Date: | 1 December 2015 | ||||||||
Dates: |
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Volume: | 10 | ||||||||
Number: | 12 | ||||||||
Article Number: | e0143943 | ||||||||
DOI: | 10.1371/journal.pone.0143943 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||
Date of first compliant deposit: | 17 December 2015 | ||||||||
Date of first compliant Open Access: | 18 December 2015 | ||||||||
Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) | ||||||||
Grant number: | BB/F022832/1 | ||||||||
Adapted As: | |||||||||
Is Part Of: | The research was funded by a grant from the Biotechnology and Biological Sciences Research Council (BBSRC) to IAC and PS (Ref: BB/F022832/1). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | ||||||||
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