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Self-assembly of temperature-responsive protein–polymer bioconjugates
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Moatsou, Dafni, Li, Jian, Ranji, Arnaz, Pitto-Barry, Anaïs, Ntai, Ioanna, Jewett, Michael C. and O’Reilly, Rachel K. (2015) Self-assembly of temperature-responsive protein–polymer bioconjugates. Bioconjugate Chemistry, 26 (9). pp. 1890-1899. doi:10.1021/acs.bioconjchem.5b00264 ISSN 1043-1802.
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Official URL: http://dx.doi.org/10.1021/acs.bioconjchem.5b00264
Abstract
We report a simple temperature-responsive bioconjugate system comprising superfolder green fluorescent protein (sfGFP) decorated with poly[(oligo ethylene glycol) methyl ether methacrylate] (PEGMA) polymers. We used amber suppression to site-specifically incorporate the non-canonical azide-functional amino acid p-azidophenylalanine (pAzF) into sfGFP at different positions. The azide moiety on modified sfGFP was then coupled using copper-catalyzed “click” chemistry with the alkyne terminus of a PEGMA synthesized by reversible addition–fragmentation chain transfer (RAFT) polymerization. The protein in the resulting bioconjugate was found to remain functionally active (i.e., fluorescent) after conjugation. Turbidity measurements revealed that the point of attachment of the polymer onto the protein scaffold has an impact on the thermoresponsive behavior of the resultant bioconjugate. Furthermore, small-angle X-ray scattering analysis showed the wrapping of the polymer around the protein in a temperature-dependent fashion. Our work demonstrates that standard genetic manipulation combined with an expanded genetic code provides an easy way to construct functional hybrid biomaterials where the location of the conjugation site on the protein plays an important role in determining material properties. We anticipate that our approach could be generalized for the synthesis of complex functional materials with precisely defined domain orientation, connectivity, and composition.
Item Type: | Journal Article | ||||||||
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Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH426 Genetics |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||||||
Library of Congress Subject Headings (LCSH): | Biomedical materials, Bioconjugates, Proteins, Polymers | ||||||||
Journal or Publication Title: | Bioconjugate Chemistry | ||||||||
Publisher: | American Chemical Society | ||||||||
ISSN: | 1043-1802 | ||||||||
Official Date: | 16 September 2015 | ||||||||
Dates: |
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Volume: | 26 | ||||||||
Number: | 9 | ||||||||
Page Range: | pp. 1890-1899 | ||||||||
DOI: | 10.1021/acs.bioconjchem.5b00264 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||
Date of first compliant deposit: | 14 January 2016 | ||||||||
Date of first compliant Open Access: | 23 February 2016 | ||||||||
Funder: | Engineering and Physical Sciences Research Council (EPSRC), University of Warwick, National Science Foundation (U.S.) (NSF), United States. Defense Advanced Research Projects Agency (DARPA), Seventh Framework Programme (European Commission) (FP7), European Research Council (ERC) | ||||||||
Grant number: | MCB-0943393 (NSF), N66001-11-1-413 (DARPA), DMR - 1108350 (NSF), FP/2007-2013 (FP7), n. 615142 (ERC), |
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