The Library
Tonoplast aquaporins facilitate lateral root emergence
Tools
Reinhardt , Hagen , Hachez , Charles , Bienert, Manuela Désirée, Beebo , Azeez , Swarup , Kamal , Voss , Ute, Bouhidel , Karim , Frigerio, Lorenzo, Schjoerring, Jan K. , Bennett , Malcolm J. and Chaumont , Francois (2016) Tonoplast aquaporins facilitate lateral root emergence. Plant Physiology, 170 (3). pp. 1640-1654. 01635.2015. doi:10.1104/pp.15.01635 ISSN 0032-0889.
|
PDF
WRAP_Frigerio_0380721-lf-210116-reinhardt_01-20-2016.pdf - Requires a PDF viewer. Download (2148Kb) | Preview |
Official URL: http://dx.doi.org/10.1104/pp.15.01635
Abstract
Aquaporins (AQPs) are water channels allowing fast and passive diffusion of water across cell membranes. It was hypothesized that AQPs contribute to cell elongation processes by allowing water influx across the plasma membrane and the tonoplast in order to maintain adequate turgor pressure. Here, we report that, in Arabidopsis thaliana, the highly abundant tonoplast AQP isoforms AtTIP1;1, AtTIP1;2 and AtTIP2;1 facilitate the emergence of new lateral root primordia (LRP). The number of lateral roots was strongly reduced in the triple tip mutant, whereas the single, double and triple tip mutants showed no or minor reduction in growth of the main root. This phenotype was due to the retardation of LRP emergence. Live cell imaging revealed that tight spatio-temporal control of TIP abundance in the tonoplast of the different LRP cells is pivotal to mediating this developmental process. While lateral root emergence is correlated to a reduction of AtTIP1;1 and AtTIP1;2 protein levels in LRPs, expression of AtTIP2;1 is specifically needed in a restricted cell population at the base, then later at the flanks, of developing LRPs. Interestingly, the LRP emergence phenotype of the triple tip mutants could be fully rescued by expressing AtTIP2;1 under its native promoter. We conclude that TIP isoforms allow the spatial and temporal fine-tuning of cellular water transport which is critically required during the highly regulated process of LRP morphogenesis and emergence.
Item Type: | Journal Article | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Subjects: | Q Science > QK Botany | ||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||
Library of Congress Subject Headings (LCSH): | Tonoplasts, Aquaporins, Roots (Botany) -- Growth | ||||||||||
Journal or Publication Title: | Plant Physiology | ||||||||||
Publisher: | American Society of Plant Biologists | ||||||||||
ISSN: | 0032-0889 | ||||||||||
Official Date: | March 2016 | ||||||||||
Dates: |
|
||||||||||
Volume: | 170 | ||||||||||
Number: | 3 | ||||||||||
Page Range: | pp. 1640-1654 | ||||||||||
Article Number: | 01635.2015 | ||||||||||
DOI: | 10.1104/pp.15.01635 | ||||||||||
Status: | Peer Reviewed | ||||||||||
Publication Status: | Published | ||||||||||
Access rights to Published version: | Open Access (Creative Commons) |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year