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Using RNA as molecular code for programming cellular function
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Kushwaha, Manish, Rostain, William, Prakash, Satya, Duncan, John and Jaramillo, Alfonso (2016) Using RNA as molecular code for programming cellular function. ACS Synthetic Biology, 5 (8). pp. 795-809. doi:10.1021/acssynbio.5b00297 ISSN 2161-5063.
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Official URL: http://dx.doi.org/10.1021/acssynbio.5b00297
Abstract
RNA is involved in a wide-range of important molecular processes in the cell, serving diverse functions: regulatory, enzymatic, and structural. Together with its ease and predictability of design, this lends it to become a useful handle for biological engineers with which to control the cellular machinery. By modifying the many RNA links in cellular processes, it is possible to re-program cells towards specific design goals. We propose that RNA can be viewed as a molecular programming language that, together with protein-based execution platforms, can be used to re-write wide ranging aspects of cellular function. In this review, we catalogue developments in the use of RNA parts, methods, and associated computational models that have contributed to the programmability of biology. We discuss how RNA part repertoires have been combined to build complex genetic circuits, and review recent applications of RNA-based parts and circuitry. We explore the future potential of RNA engineering and posit that RNA programmability is an important resource for firmly establishing an era of rationally designed synthetic biology.
Item Type: | Journal Article | ||||||||
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Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
Library of Congress Subject Headings (LCSH): | RNA, Molecular biology, RNA -- Structure | ||||||||
Journal or Publication Title: | ACS Synthetic Biology | ||||||||
Publisher: | American Chemical Society | ||||||||
ISSN: | 2161-5063 | ||||||||
Official Date: | 21 March 2016 | ||||||||
Dates: |
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Volume: | 5 | ||||||||
Number: | 8 | ||||||||
Page Range: | pp. 795-809 | ||||||||
DOI: | 10.1021/acssynbio.5b00297 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||
Date of first compliant deposit: | 1 April 2016 | ||||||||
Date of first compliant Open Access: | 21 March 2017 | ||||||||
Funder: | Engineering and Physical Sciences Research Council (EPSRC), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), European Commission (EC) | ||||||||
Grant number: | EPSRC-BBSRC BB/M017982/1, FP7-ICT-FET 610730, FP7-KBBE 613745 |
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