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The structure of the core NuRD repression complex provides insights into its interaction with chromatin
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Millard, Christopher J., Varma, Niranjan, Saleh, Almutasem, Morris, Kyle L., Watson, Peter J., Bottrill, Andrew R., Fairall, Louise, Smith, Corinne J. and Schwabe, John W. R. (2016) The structure of the core NuRD repression complex provides insights into its interaction with chromatin. eLife, 5 . e13941. doi:10.7554/eLife.13941 ISSN 2050-084X.
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Official URL: http://dx.doi.org/10.7554/eLife.13941
Abstract
The NuRD complex is a multi-protein transcriptional corepressor that couples histone deacetylase and ATP-dependent chromatin remodelling activities. The complex regulates the higher-order structure of chromatin, and has important roles in the regulation of gene expression, DNA damage repair and cell differentiation. HDACs 1 and 2 are recruited by the MTA1 corepressor to form the catalytic core of the complex. The histone chaperone protein RBBP4, has previously been shown to bind to the carboxy-terminal tail of MTA1. We show that MTA1 recruits a second copy of RBBP4. The crystal structure reveals an extensive interface between MTA1 and RBBP4. An EM structure, supported by SAXS and crosslinking, reveals the architecture of the dimeric HDAC1:MTA1:RBBP4 assembly which forms the core of the NuRD complex. We find evidence that in this complex RBBP4 mediates interaction with histone H3 tails, but not histone H4, suggesting a mechanism for recruitment of the NuRD complex to chromatin
Item Type: | Journal Article | ||||||||
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Subjects: | Q Science > QH Natural history | ||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
Library of Congress Subject Headings (LCSH): | Chromatin, Histones | ||||||||
Journal or Publication Title: | eLife | ||||||||
Publisher: | eLife Sciences Publications Ltd. | ||||||||
ISSN: | 2050-084X | ||||||||
Official Date: | 21 April 2016 | ||||||||
Dates: |
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Volume: | 5 | ||||||||
Number of Pages: | 21 | ||||||||
Article Number: | e13941 | ||||||||
DOI: | 10.7554/eLife.13941 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||
Funder: | Wellcome Trust (London, England), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Royal Society (Great Britain). Wolfson Research Merit Award (RSWRMA) | ||||||||
Grant number: | WT100237 (WT), BB/J009598/1 (BBSRC), 055663/Z/98/Z (WT), BB/K003461/1 (BBSRC) |
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