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Immuno-gold labeling of Drosophila follicles for transmission electron microscopy
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Tsapras, Panagiotis , Sagona, Antonia P. and Nezis, Ioannis P. (2016) Immuno-gold labeling of Drosophila follicles for transmission electron microscopy. In: Oogenesis : Methods and Protocols. Methods in Molecular Biology, 1457 . Springer, pp. 97-103. ISBN 9781493937936
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Official URL: http://www.springer.com/series/7651
Abstract
Detecting the localization of cellular components using gold nanoparticles has come to offer tremendous advantages in cell biology, allowing for the high resolution imaging of the cellular organization at the subcellular level. This is further aided by the breakthroughs in the cryopreparation of samples, which focus at the retention of antigenicity in efforts to mirror the native state of the tissues and cells as closely as possible. Herein, we describe the methodology for immuno-gold labeling of Drosophila follicles, following preparation of the samples using the Tokuyasu method for ultracryosectioning.
Item Type: | Book Item | ||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
Series Name: | Methods in Molecular Biology | ||||||
Journal or Publication Title: | Methods in Molecular Biology | ||||||
Publisher: | Springer | ||||||
ISBN: | 9781493937936 | ||||||
ISSN: | 1064-3745 | ||||||
Book Title: | Oogenesis : Methods and Protocols | ||||||
Official Date: | June 2016 | ||||||
Dates: |
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Volume: | 1457 | ||||||
Page Range: | pp. 97-103 | ||||||
DOI: | 10.1007/978-1-4939-3795-0_5 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Date of first compliant deposit: | 13 June 2016 | ||||||
Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) | ||||||
Grant number: | BB/L006324/1 |
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