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The biological properties of Shiga-like toxin I
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Burgess, Beverley Jane (1993) The biological properties of Shiga-like toxin I. PhD thesis, University of Warwick.
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Official URL: http://webcat.warwick.ac.uk/record=b1415243~S1
Abstract
Shiga toxin and the Escherichia coli Shiga-like toxins (SLTs) are type 2 ribosome inactivating proteins (RIPs), exhibiting a specific RNA N-glycosidase activity comparable to that of the plant toxin ricin (Endo et al. 1988). Not only do they possess the same enzymatic activity as a plant toxin, but on examination at the molecular level, they may be seen to be classic bipartite toxins. The present study examines in detail the RNA N-glycosidase activity of Shiga-like toxin I (SLT I) ie, the ribosomes susceptibility to the toxin, of a range the exact of different eukaryotic site of SLT I-catalysed depurination of yeast ribosomal RNA and the kinetics of such depurination. The cytotoxic effect of SLT I on Vero cells is also examined. In addition to its characterisation and subsequent comparison to ricin, an attempt has been made to correlate certain structure - function aspects of SLT I. By structural comparison with other bacterial toxins it has been proposed that proteolytic cleavage with thin an arginine rich, trypsin sensitive, disulphide-bonded loop structure is essential for expression of cytotoxicity. Examination of the enzymatic activity and cytotoxicity of a 'protease insensitive' mutant SLT I A subunit (SLT lA) generated in the present study has led to the conclusion tha t proteolytic processing wi thin this disulphide-bonded loop is not essential, but that processing may occur at alternative sites within SLT lA to compensate for loss cleavage at the proposed target sites.
Item Type: | Thesis (PhD) | ||||
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Subjects: | Q Science > QR Microbiology | ||||
Library of Congress Subject Headings (LCSH): | Verocytotoxins | ||||
Official Date: | 1993 | ||||
Dates: |
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Institution: | University of Warwick | ||||
Theses Department: | Department of Biological Sciences | ||||
Thesis Type: | PhD | ||||
Publication Status: | Unpublished | ||||
Supervisor(s)/Advisor: | Roberts, L. M. (Lynne M.) | ||||
Format of File: | |||||
Extent: | 367 leaves : illustrations | ||||
Language: | eng |
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