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Human immunodeficiency virus type 2 (HIV-2) Gag is trafficked in an AP-3 and AP-5 dependent manner
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Alford, Justine E., Marongiu, Michela, Watkins, Gemma L. and Anderson, Emma C. (2016) Human immunodeficiency virus type 2 (HIV-2) Gag is trafficked in an AP-3 and AP-5 dependent manner. PLoS One, 11 (7). e0158941. doi:10.1371/journal.pone.0158941 ISSN 1932-6203.
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Official URL: http://dx.doi.org/10.1371/journal.pone.0158941
Abstract
Although human immunodeficiency virus (HIV) types 1 and 2 are closely related lentiviruses with similar replication cycles, HIV-2 infection is associated with slower progression to AIDS, a higher proportion of long term non-progressors, and lower rates of transmission than HIV-1, likely as a consequence of a lower viral load during HIV-2 infection. A mechanistic explanation for the differential viral load remains unclear but knowledge of differences in particle production between HIV-1 and HIV-2 may help to shed light on this issue. In contrast to HIV-1, little is known about the assembly of HIV-2 particles, and the trafficking of HIV-2 Gag, the structural component of the virus, within cells. We have established that HIV-2 Gag accumulates in intracellular CD63 positive compartments, from which it may be delivered or recycled to the cell surface, or degraded. HIV-2 particle release was dependent on the adaptor protein complex AP-3 and the newly identified AP-5 complex, but much less so on AP-1. In contrast, HIV-1 particle release required AP-1 and AP-3, but not AP-5. AP-2, an essential component of clathrin-mediated endocytosis, which was previously shown to be inhibitory to HIV-1 particle release, had no effect on HIV-2. The differential requirement for adaptor protein complexes confirmed that HIV-1 and HIV-2 Gag have distinct cellular trafficking pathways, and that HIV-2 particles may be more susceptible to degradation prior to release.
| Item Type: | Journal Article | ||||||||
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| Subjects: | Q Science > QR Microbiology > QR355 Virology | ||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
| Library of Congress Subject Headings (LCSH): | HIV (Viruses) | ||||||||
| Journal or Publication Title: | PLoS One | ||||||||
| Publisher: | Public Library of Science | ||||||||
| ISSN: | 1932-6203 | ||||||||
| Official Date: | 8 July 2016 | ||||||||
| Dates: |
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| Volume: | 11 | ||||||||
| Number: | 7 | ||||||||
| Article Number: | e0158941 | ||||||||
| DOI: | 10.1371/journal.pone.0158941 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||||
| Date of first compliant deposit: | 19 July 2016 | ||||||||
| Date of first compliant Open Access: | 19 July 2016 | ||||||||
| Funder: | Medical Research Council (Great Britain) (MRC) | ||||||||
| Grant number: | G0701220 (MRC) |
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