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Assessing Illumina technology for the high-throughput sequencing of bacteriophage genomes
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Rihtman, Branko, Meaden, Sean, Clokie, Martha R. J., Koskella, Britt and Millard, Andrew D. (2016) Assessing Illumina technology for the high-throughput sequencing of bacteriophage genomes. PeerJ, 4 . pp. 1-21. e2055. doi:10.7717/peerj.2055 ISSN 2167-8359.
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Official URL: http://dx.doi.org/10.7717/peerj.2055
Abstract
Bacteriophages are the most abundant biological entities on the planet, playing crucial roles in the shaping of bacterial populations. Phages have smaller genomes than their bacterial hosts, yet there are currently fewer fully sequenced phage than bacterial genomes. We assessed the suitability of Illumina technology for high-throughput sequencing and subsequent assembly of phage genomes. In silico datasets reveal that 30× coverage is sufficient to correctly assemble the complete genome of ~98.5% of known phages, with experimental data confirming that the majority of phage genomes can be assembled at 30× coverage. Furthermore, in silico data demonstrate it is possible to co-sequence multiple phages from different hosts, without introducing assembly errors.
Item Type: | Journal Article | ||||||||
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Subjects: | Q Science > QR Microbiology | ||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Library of Congress Subject Headings (LCSH): | Bacteriophages -- Genetics | ||||||||
Journal or Publication Title: | PeerJ | ||||||||
Publisher: | PeerJ, Ltd. | ||||||||
ISSN: | 2167-8359 | ||||||||
Official Date: | 1 June 2016 | ||||||||
Dates: |
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Volume: | 4 | ||||||||
Number of Pages: | 21 | ||||||||
Page Range: | pp. 1-21 | ||||||||
Article Number: | e2055 | ||||||||
DOI: | 10.7717/peerj.2055 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||
Date of first compliant deposit: | 5 August 2016 | ||||||||
Date of first compliant Open Access: | 5 August 2016 | ||||||||
Funder: | University of Warwick Chancellor’s International Scholarship |
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