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Multifaceted studies of the DNA interactions and in vitro cytotoxicity of anticancer polyaromatic platinum(II) complexes
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Pages, Benjamin J., Sakoff, Jennette, Gilbert, Jayne, Rodger, Alison, Chmel, Nikola Paul, Jones, Nykola C., Kelly, Sharon M., Ang, Dale L. and Aldrich-Wright, Janice R. (2016) Multifaceted studies of the DNA interactions and in vitro cytotoxicity of anticancer polyaromatic platinum(II) complexes. Chemistry - A European Journal, 22 (26). pp. 8943-8954. doi:10.1002/chem.201601221 ISSN 0947-6539.
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Official URL: http://dx.doi.org/10.1002/chem.201601221
Abstract
This study reports a detailed biophysical analysis of the DNA binding and cytotoxicity of six platinum complexes (PCs). They are of the type [Pt(PL)(SS-dach)]Cl 2, where PL is a polyaromatic ligand and SS-dach is 1S,2S-diaminocyclohexane. The DNA binding of these complexes was investigated using six techniques including ultraviolet and fluorescence spectroscopy, linear dichroism, synchrotron radiation circular dichroism, isothermal titration calorimetry and mass spectrometry. This portfolio of techniques has not been extensively used to study the interactions of such complexes previously; each assay provided unique insight. The in vitro cytotoxicity of these compounds was studied in ten cell lines and compared to the effects of their R,R enantiomers; activity was very high in Du145 and SJ-G2 cells, with some submicromolar IC50 values. In terms of both DNA affinity and cytotoxicity, complexes of 5,6-dimethyl-1,10-phenanthroline and 2,2’-bipyridine exhibited the greatest and least activity, respectively, suggesting that there is some correlation between DNA binding and cytotoxicity.
Item Type: | Journal Article | ||||||||||
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Subjects: | Q Science > QD Chemistry Q Science > QP Physiology R Medicine > RC Internal medicine R Medicine > RS Pharmacy and materia medica |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||||||||
Library of Congress Subject Headings (LCSH): | DNA, Antineoplastic agents, Spectrum analysis | ||||||||||
Journal or Publication Title: | Chemistry - A European Journal | ||||||||||
Publisher: | Wiley-Blackwell Publishing Ltd. | ||||||||||
ISSN: | 0947-6539 | ||||||||||
Official Date: | 20 June 2016 | ||||||||||
Dates: |
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Volume: | 22 | ||||||||||
Number: | 26 | ||||||||||
Page Range: | pp. 8943-8954 | ||||||||||
DOI: | 10.1002/chem.201601221 | ||||||||||
Status: | Peer Reviewed | ||||||||||
Publication Status: | Published | ||||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||||
Date of first compliant deposit: | 13 August 2016 | ||||||||||
Date of first compliant Open Access: | 24 May 2017 | ||||||||||
Funder: | Australian Synchrotron, University of Western Sydney (UWS), Australia. Department of Education, Science, and Training |
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