Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

Multifaceted studies of the DNA interactions and in vitro cytotoxicity of anticancer polyaromatic platinum(II) complexes

Tools
- Tools
+ Tools

Pages, Benjamin J., Sakoff, Jennette, Gilbert, Jayne, Rodger, Alison, Chmel, Nikola Paul, Jones, Nykola C., Kelly, Sharon M., Ang, Dale L. and Aldrich-Wright, Janice R. (2016) Multifaceted studies of the DNA interactions and in vitro cytotoxicity of anticancer polyaromatic platinum(II) complexes. Chemistry - A European Journal, 22 (26). pp. 8943-8954. doi:10.1002/chem.201601221 ISSN 0947-6539.

[img] PDF
WRAP_binding_studies_paper_colour_190416.pdf - Accepted Version - Requires a PDF viewer.

Download (3375Kb)
Official URL: http://dx.doi.org/10.1002/chem.201601221

Request Changes to record.

Abstract

This study reports a detailed biophysical analysis of the DNA binding and cytotoxicity of six platinum complexes (PCs). They are of the type [Pt(PL)(SS-dach)]Cl 2, where PL is a polyaromatic ligand and SS-dach is 1S,2S-diaminocyclohexane. The DNA binding of these complexes was investigated using six techniques including ultraviolet and fluorescence spectroscopy, linear dichroism, synchrotron radiation circular dichroism, isothermal titration calorimetry and mass spectrometry. This portfolio of techniques has not been extensively used to study the interactions of such complexes previously; each assay provided unique insight. The in vitro cytotoxicity of these compounds was studied in ten cell lines and compared to the effects of their R,R enantiomers; activity was very high in Du145 and SJ-G2 cells, with some submicromolar IC50 values. In terms of both DNA affinity and cytotoxicity, complexes of 5,6-dimethyl-1,10-phenanthroline and 2,2’-bipyridine exhibited the greatest and least activity, respectively, suggesting that there is some correlation between DNA binding and cytotoxicity.

Item Type: Journal Article
Subjects: Q Science > QD Chemistry
Q Science > QP Physiology
R Medicine > RC Internal medicine
R Medicine > RS Pharmacy and materia medica
Divisions: Faculty of Science, Engineering and Medicine > Science > Chemistry
Library of Congress Subject Headings (LCSH): DNA, Antineoplastic agents, Spectrum analysis
Journal or Publication Title: Chemistry - A European Journal
Publisher: Wiley-Blackwell Publishing Ltd.
ISSN: 0947-6539
Official Date: 20 June 2016
Dates:
DateEvent
20 June 2016Published
24 May 2016Available
13 April 2016Accepted
15 March 2016Submitted
Volume: 22
Number: 26
Page Range: pp. 8943-8954
DOI: 10.1002/chem.201601221
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
Date of first compliant deposit: 13 August 2016
Date of first compliant Open Access: 24 May 2017
Funder: Australian Synchrotron, University of Western Sydney (UWS), Australia. Department of Education, Science, and Training

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics

twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us