Pages, Benjamin J., Sakoff, Jennette, Gilbert, Jayne, Rodger, Alison, Chmel, Nikola Paul, Jones, Nykola C., Kelly, Sharon M., Ang, Dale L. and Aldrich-Wright, Janice R. (2016) Multifaceted studies of the DNA interactions and in vitro cytotoxicity of anticancer polyaromatic platinum(II) complexes. Chemistry - A European Journal, 22 (26). pp. 8943-8954. doi:10.1002/chem.201601221

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Abstract

This study reports a detailed biophysical analysis of the DNA binding and cytotoxicity of six platinum complexes (PCs). They are of the type [Pt(PL)(SS-dach)]Cl 2, where PL is a polyaromatic ligand and SS-dach is 1S,2S-diaminocyclohexane. The DNA binding of these complexes was investigated using six techniques including ultraviolet and fluorescence spectroscopy, linear dichroism, synchrotron radiation circular dichroism, isothermal titration calorimetry and mass spectrometry. This portfolio of techniques has not been extensively used to study the interactions of such complexes previously; each assay provided unique insight. The in vitro cytotoxicity of these compounds was studied in ten cell lines and compared to the effects of their R,R enantiomers; activity was very high in Du145 and SJ-G2 cells, with some submicromolar IC50 values. In terms of both DNA affinity and cytotoxicity, complexes of 5,6-dimethyl-1,10-phenanthroline and 2,2’-bipyridine exhibited the greatest and least activity, respectively, suggesting that there is some correlation between DNA binding and cytotoxicity.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry Q Science > QP Physiology R Medicine > RC Internal medicine R Medicine > RS Pharmacy and materia medica
Divisions:	Faculty of Science > Chemistry
Library of Congress Subject Headings (LCSH):	DNA, Antineoplastic agents, Spectrum analysis
Journal or Publication Title:	Chemistry - A European Journal
Publisher:	Wiley-Blackwell Publishing Ltd.
ISSN:	0947-6539
Official Date:	20 June 2016
Dates:	Date Event 20 June 2016 Published 24 May 2016 Available 13 April 2016 Accepted 15 March 2016 Submitted
Date of first compliant deposit:	13 August 2016
Volume:	22
Number:	26
Page Range:	pp. 8943-8954
DOI:	10.1002/chem.201601221
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Australian Synchrotron, University of Western Sydney (UWS), Australia. Department of Education, Science, and Training

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