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Mechanistic modeling of a rewritable recombinase addressable data module
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Bowyer, Jack E., Zhao, Jia, Subsoontorn, Pakpoom, Wong, Wilson, Rosser, Susan and Bates, Declan (2016) Mechanistic modeling of a rewritable recombinase addressable data module. IEEE Transactions on Biomedical Circuits and Systems, 10 (6). pp. 1161-1170. doi:10.1109/TBCAS.2016.2526668 ISSN 1940-9990.
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Official URL: http://dx.doi.org/10.1109/TBCAS.2016.2526668
Abstract
Many of the most important applications predicted to arise from Synthetic Biology will require engineered cellular memory with the capability to store data in a rewritable and reversible manner upon induction by transient stimuli. DNA recombination provides an ideal platform for cellular data storage and has allowed the development of a rewritable recombinase addressable data (RAD) module, capable of efficient data storage within a chromosome. Here, we develop the first detailed mechanistic model of DNA recombination, and validate it against a new set of in vitro data on recombination efficiencies across a range of different concentrations of integrase and gp3. Investigation of in vivo recombination dynamics using our model reveals the importance of fully accounting for all mechanistic features of DNA recombination in order to accurately predict the effect of different switching strategies on RAD module performance, and highlights its usefulness as a design tool for building future synthetic circuitry.
Item Type: | Journal Article | ||||||||||
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Subjects: | Q Science > QH Natural history > QH426 Genetics | ||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Engineering > Engineering | ||||||||||
Library of Congress Subject Headings (LCSH): | Recombinant DNA -- Mathematical models, Synthetic biology | ||||||||||
Journal or Publication Title: | IEEE Transactions on Biomedical Circuits and Systems | ||||||||||
Publisher: | Institute of Electrical and Electronics Engineers | ||||||||||
ISSN: | 1940-9990 | ||||||||||
Official Date: | 24 May 2016 | ||||||||||
Dates: |
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Volume: | 10 | ||||||||||
Number: | 6 | ||||||||||
Page Range: | pp. 1161-1170 | ||||||||||
DOI: | 10.1109/TBCAS.2016.2526668 | ||||||||||
Status: | Peer Reviewed | ||||||||||
Publication Status: | Published | ||||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||||
Date of first compliant deposit: | 31 August 2016 | ||||||||||
Date of first compliant Open Access: | 31 August 2016 | ||||||||||
Funder: | Engineering and Physical Sciences Research Council (EPSRC) | ||||||||||
Grant number: | EP/K034359/1, EP/H019154/1 |
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