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Esterase EstK from Pseudomonas putida mt-2 : an enantioselective acetylesterase with activity for deacetylation of xylan and poly(vinylacetate)
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Millar, Robert, Rahmanpour, Rahman, Wei Jia Yuan, Eugenie, White, Catharine and Bugg, Tim (2017) Esterase EstK from Pseudomonas putida mt-2 : an enantioselective acetylesterase with activity for deacetylation of xylan and poly(vinylacetate). Biotechnology and Applied Biochemistry, 64 (6). pp. 803-809. ISSN 0885-4513.
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WRAP_9970029-ch-041016-esterase_ms_2016_v5_unmarked.pdf - Accepted Version - Requires a PDF viewer. Download (690Kb) |
Official URL: http://dx.doi.org/10.1002/bab.1536
Abstract
An extracellular esterase gene estK was identified in Pseudomonas putida mt-2, and was overexpressed to high levels in Escherichia coli. The recombinant EstK enzyme was purified and characterised kinetically against p-nitrophenyl ester and other aryl-alkyl ester substrates, and was found to be selective for hydrolysis of acetyl ester substrates, with high activity for p-nitrophenyl acetate (kcat 5.5 s-1, KM 285 µM). Recombinant EstK was found to catalyse deacetylation of acetylated beech xylan, indicating a possible in vivo function for this enzyme, and partial deacetylation of a synthetic polymer, poly(vinylacetate). EstK was found to catalyse enantioselective hydrolysis of racemic 1-phenylethyl acetate, generating 1R-phenylethanol with an enantiomeric excess of 80.4%.
Item Type: | Journal Article | ||||||||||
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Subjects: | Q Science > QP Physiology | ||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||||||||
Library of Congress Subject Headings (LCSH): | Esterases, Pseudomonas | ||||||||||
Journal or Publication Title: | Biotechnology and Applied Biochemistry | ||||||||||
Publisher: | John Wiley & Sons, Inc. | ||||||||||
ISSN: | 0885-4513 | ||||||||||
Official Date: | November 2017 | ||||||||||
Dates: |
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Volume: | 64 | ||||||||||
Number: | 6 | ||||||||||
Page Range: | pp. 803-809 | ||||||||||
Status: | Peer Reviewed | ||||||||||
Publication Status: | Published | ||||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||||
Date of first compliant deposit: | 6 October 2016 | ||||||||||
Date of first compliant Open Access: | 3 October 2017 | ||||||||||
Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) | ||||||||||
Grant number: | BB/M025772/1 (BBSRC) | ||||||||||
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