Kostalova, T., Lestinova, T., Maia, C., Sumova, P., Vlkova, M., Willen, L., Polanska, N., Fiorentino, E., Scalone, A., Oliva, G., Veronesi, F., Cristóvão, J. M., Courtenay, Orin, Campino, L., Gradoni, L., Gramiccia, M. and Volf, P. (2017) The recombinant protein rSP03B is a valid antigen for screening dog exposure to Phlebotomus perniciosusacross foci of canine leishmaniasis. Medical and Veterinary Entomology, 31 (1). pp. 88-93. doi:10.1111/mve.12192 ISSN 0269-283X.

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Abstract

The frequency of sandfly–host contacts can be measured by host antibody levels against sandfly salivary proteins. Recombinant salivary proteins are suggested to represent a valid replacement for salivary gland homogenate (SGH); however, it is necessary to prove that such antigens are recognized by antibodies against various populations of the same species. Phlebotomus perniciosus (Diptera: Psychodidae) is the main vector of Leishmania infantum (Trypanosomatida: Trypanosomatidae) in southwest Europe and is widespread from Portugal to Italy. In this study, sera were sampled from naturally exposed dogs from distant regions, including Campania (southern Italy), Umbria (central Italy) and the metropolitan Lisbon region (Portugal), where P. perniciosus is the unique or principal vector species. Sera were screened for anti-P. perniciosus antibodies using SGH and 43-kDa yellow-related recombinant protein (rSP03B). A robust correlation between antibodies recognizing SGH and rSP03B was detected in all regions, suggesting substantial antigenic cross-reactivity among different P. perniciosus populations. No significant differences in this relationship were detected between regions. Moreover, rSP03B and the native yellow-related protein were shown to share similar antigenic epitopes, as canine immunoglobulin G (IgG) binding to the native protein was inhibited by pre-incubation with the recombinant form. These findings suggest that rSP03B should be regarded as a universal marker of sandfly exposure throughout the geographical distribution of P. perniciosus.

Item Type:	Journal Article
Alternative Title:
Subjects:	Q Science > QL Zoology S Agriculture > SF Animal culture
Divisions:	Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH):	Leishmania, Leishmaniasis, Dogs -- Parasites, Phlebotomus, Mediterranean Region, Salivary proteins, Sand flies
Journal or Publication Title:	Medical and Veterinary Entomology
Publisher:	Wiley-Blackwell Publishing, Inc.
ISSN:	0269-283X
Official Date:	March 2017
Dates:	Date Event March 2017 Published 8 October 2016 Available 5 June 2016 Accepted 1 February 2016 Submitted
Volume:	31
Number:	1
Page Range:	pp. 88-93
DOI:	10.1111/mve.12192
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Open Access (Creative Commons)
Date of first compliant deposit:	19 October 2016
Date of first compliant Open Access:	19 October 2016
Funder:	Univerzita Karlova. Grantové agentury (GAUK), Seventh Framework Programme (European Commission) (FP7), Horizon 2020 (European Commission) (H2020), Marie Skłodowska-Curie Actions, Portugal. Ministério da Ciência e da Tecnologia‏ [Foundation for Science and Technology]
Grant number:	1642314/201 (GAUK ) 642609 (Marie Skłodowska-Curie), SFRH/BPD/44082/200 (Portugal)

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