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Quantitation of plasma thiamine, related metabolites and plasma protein oxidative damage markers in children with autism spectrum disorder and healthy controls
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Anwar, Attia, Marini, Marina, Abruzzo, Provvidenza Maria, Bolotta, Alessandra, Ghezzo, Alessandro, Visconti, Paola, Thornalley, Paul J. and Rabbani, Naila (2016) Quantitation of plasma thiamine, related metabolites and plasma protein oxidative damage markers in children with autism spectrum disorder and healthy controls. Free Radical Research, 50 (Supplement 1). S85-S90 . doi:10.1080/10715762.2016.1239821 ISSN 1071-5762.
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Official URL: http://dx.doi.org/10.1080/10715762.2016.1239821
Abstract
AIMS/HYPOTHESIS:
To assess thiamine and related metabolite status by analysis of plasma and urine in autistic children and healthy controls, correlations to clinical characteristics and link to plasma protein markers of oxidative damage.
METHODS:
27 children with autism (21 males and 6 females) and 21 (15 males and 6 females) age-matched healthy control children were recruited. The concentration of thiamine and related phosphorylated metabolites in plasma and urine and plasma protein content of dityrosine, N-formylkynurenine and 3-nitrotyrosine was determined.
RESULTS:
Plasma thiamine and thiamine monophosphate concentrations were similar in both study groups (median [lower-upper quartile]): autistic children - 6.60 nM (4.48-8.91) and 7.00 nM (5.51-8.55), and healthy controls - 6.82 nM (4.47-7.02) and 6.82 nM (5.84-8.91), respectively. Thiamine pyrophosphate (TPP) was decreased 24% in autistic children compared to healthy controls: 6.82 nM (5.81-8.52) versus 9.00 nM (8.41-10.71), p < .01. Urinary excretion of thiamine and fractional renal clearance of thiamine did not change between the groups. No correlation was observed between clinical markers and the plasma and urine thiamine concentration. Plasma protein dityrosine content was increased 88% in ASD. Other oxidative markers were unchanged.
CONCLUSIONS/INTERPRETATION:
Autistic children had normal plasma and urinary thiamine levels whereas plasma TPP concentration was decreased. The latter may be linked to abnormal tissue handling and/or absorption from gut microbiota of TPP which warrants further investigation. Increased plasma protein dityrosine may reflect increased dual oxidase activity in response to change in mucosal immunity and host-microbe homeostasis.
| Item Type: | Journal Article | ||||||||||
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| Divisions: | Faculty of Science, Engineering and Medicine > Research Centres > Warwick Systems Biology Centre Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Journal or Publication Title: | Free Radical Research | ||||||||||
| Publisher: | Taylor & Francis Inc. | ||||||||||
| ISSN: | 1071-5762 | ||||||||||
| Official Date: | 2 November 2016 | ||||||||||
| Dates: |
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| Volume: | 50 | ||||||||||
| Number: | Supplement 1 | ||||||||||
| Page Range: | S85-S90 | ||||||||||
| DOI: | 10.1080/10715762.2016.1239821 | ||||||||||
| Status: | Peer Reviewed | ||||||||||
| Publication Status: | Published | ||||||||||
| Access rights to Published version: | Open Access (Creative Commons) |
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