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Human kinetochores are swivel joints that mediate microtubule attachments.
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Smith, Chris A., McAinsh, Andrew D. and Burroughs, Nigel John (2016) Human kinetochores are swivel joints that mediate microtubule attachments. eLife, 5 . e16159. doi:10.7554/eLife.16159 ISSN 2050-084X.
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Official URL: http://dx.doi.org/10.7554/eLife.16159
Abstract
Chromosome segregation is a mechanical process that requires assembly of the mitotic spindle - a dynamic microtubule-based force-generating machine. Connections to this spindle are mediated by sister kinetochore pairs, that form dynamic end-on attachments to microtubules emanating from opposite spindle poles. This bi-orientation generates forces that have been reported to stretch the kinetochore itself, which has been suggested to stabilise attachment and silence the spindle checkpoint. We reveal using three dimensional tracking that the outer kinetochore domain can swivel around the inner kinetochore/centromere, which results in large reductions in intra-kinetochore distance (delta) when viewed in lower dimensions. We show that swivel provides a mechanical flexibility that enables kinetochores at the periphery of the spindle to engage microtubules. Swivel reduces as cells approach anaphase, suggesting an organisational change linked to checkpoint satisfaction and/or obligatory changes in kinetochore mechanochemistry may occur before dissolution of sister chromatid cohesion.
| Item Type: | Journal Article | ||||||||
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| Subjects: | Q Science > QH Natural history Q Science > QL Zoology Q Science > QM Human anatomy |
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| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Chromosomes, Chromosome replication, Joints, Microtubules | ||||||||
| Journal or Publication Title: | eLife | ||||||||
| Publisher: | eLife Sciences Publications Ltd. | ||||||||
| ISSN: | 2050-084X | ||||||||
| Official Date: | 2 September 2016 | ||||||||
| Dates: |
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| Volume: | 5 | ||||||||
| Article Number: | e16159 | ||||||||
| DOI: | 10.7554/eLife.16159 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||||
| Date of first compliant deposit: | 14 January 2017 | ||||||||
| Date of first compliant Open Access: | 9 January 2019 | ||||||||
| Funder: | Wellcome Trust (London, England), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Engineering and Physical Sciences Research Council (EPSRC) |
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